Wu Changzhu, Zhai Ning, Guan Chao, Ji Wenyu
Department of Otorhinolaryngology, the First Affiliated Hospital of China Medical University, Beijing, China.
Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2006 May;20(9):393-5.
To study whether Integrin linked kinase (ILK) is involved in the development and progression of laryngeal squamous cell carcinomas (LSCC).
We examined the expression of ILK in 64 LSCC (LSCC group) and 10 normal laryngeal mucosa (control group) using reverse transcription polymerase chain reaction (RT-PCR) and analyzed the relationship with clinic pathological parameters.
The expression of ILK mRNA was significantly higher in 64 LSCC tissue (0. 644 +/- 0. 098) than in normal laryngeal mucosa tissues (0.032 +/- 0.026, P <0.05). The expression of ILK mRNA in LSCC with the metastasis of lymph nodes (0. 867 +/- 0.247) was significantly higher than those without metastasis (0.389 +/- 0.199, P <0.05). With the development of clinical T-stages of LSCC, the expression of ILK mRNA in LSCC increases. There was no significant difference between different clinical stage groups (P > 0.05).
These results suggest that ILK may participate not only in LSCC but also in invasion and metastasis of LSCC. ILK might be a novel molecular marker for prognosticating metastases and prognosis of LSCC.
研究整合素连接激酶（ILK）是否参与喉鳞状细胞癌（LSCC）的发生发展过程。
采用逆转录聚合酶链反应（RT-PCR）检测64例喉鳞状细胞癌组织（LSCC组）和10例正常喉黏膜组织（对照组）中ILK的表达情况，并分析其与临床病理参数的关系。
64例喉鳞状细胞癌组织中ILK mRNA的表达水平（0.644±0.098）显著高于正常喉黏膜组织（0.032±0.026，P<0.05）。有淋巴结转移的喉鳞状细胞癌组织中ILK mRNA的表达水平（0.867±0.247）显著高于无淋巴结转移者（0.389±0.199，P<0.05）。随着喉鳞状细胞癌临床T分期的增加，ILK mRNA的表达水平升高。不同临床分期组之间差异无统计学意义（P>0.05）。
这些结果提示ILK不仅可能参与喉鳞状细胞癌的发生，还可能参与其侵袭和转移过程。ILK可能是预测喉鳞状细胞癌转移和预后的一个新的分子标志物。
