Zhong Ding-rong, Ling Qing, Shi Xiao-hua, Liang Zhi-yong, Liu Tong-hua
Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100730, China.
Zhonghua Bing Li Xue Za Zhi. 2012 Jun;41(6):361-5. doi: 10.3760/cma.j.issn.0529-5807.2012.06.001.
To investigate the differences between primary mediastinal B-cell lymphoma (PMBCL) and non-mediastinal conventional diffuse large B-cell common lymphoma (DLBCL) in immunoglobulin gene rearrangement and EB virus infections.
Twenty cases of PMBCL and 30 cases of non-mediastinal DLBCL were collected from September, 2000 to May, 2011. Pathological data were retrospectively analysed. Immunoglobulin heavy chain and light chain gene rearrangements and EBER in-situ hybridization were performed.
Six of 20 cases of PMBCL showed monoclonal gene rearrangement, all of which were weakly detected. Twenty-seven of 30 cases of ordinary diffuse large B-cell lymphoma showed monoclonal gene rearrangement, which were strongly detected (90.0%). Only 1 of 20 cases PMBCL and 2 of 30 cases of DLBCL were positive for EBER in-situ hybridization.
The detection rate of immunoglobulin gene rearrangement is significantly lower in PMBCL than that of non-mediastinal DLBCL. However, EB virus infection rates are very low in both types of lymphomas.
探讨原发性纵隔B细胞淋巴瘤（PMBCL）与非纵隔型经典弥漫大B细胞淋巴瘤（DLBCL）在免疫球蛋白基因重排及EB病毒感染方面的差异。
收集2000年9月至2011年5月间20例PMBCL及30例非纵隔型DLBCL病例，对其病理资料进行回顾性分析，并行免疫球蛋白重链和轻链基因重排检测及EBER原位杂交。
20例PMBCL中有6例显示单克隆基因重排，均为弱表达；30例普通弥漫大B细胞淋巴瘤中有27例显示单克隆基因重排，且表达较强（90.0%）。20例PMBCL中仅1例、30例DLBCL中仅2例EBER原位杂交呈阳性。
PMBCL中免疫球蛋白基因重排的检出率明显低于非纵隔型DLBCL。然而，两种类型淋巴瘤的EB病毒感染率均很低。
