Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, 518 Ziyue Road, Shanghai 200241, China.
Parasitol Res. 2012 Dec;111(6):2317-24. doi: 10.1007/s00436-012-3086-4. Epub 2012 Aug 30.
Cytokine-induced apoptosis inhibitor (CIAP) is a novel antiapoptotic molecule, which is different to inhibitor of apoptosis protein or B-cell lymphoma 2. CIAP was originally identified as a molecule that conferred resistance to apoptosis induced by growth factor starvation. However, it remains to be undercharacterized in schistosomes. Here, we molecularly characterize a novel cytokine-induced apoptosis inhibitor from Schistosoma japonicum (SjCIAP). The transcription of the SjCIAP occurred at all of developmental stages investigated including eggs, cercariae, schistosomula, and adult schistosomes. Functional assay indicated that the SjCIAP could inhibit caspase activity in either human cell lines or schistosome lysates. Our preliminary results suggest that the SjCIAP may play important roles in parasitic living and development by regulating apoptosis, and drug target of SjCIAP might be a potential for schistosomiasis control.
细胞因子诱导的凋亡抑制剂 (CIAP) 是一种新型的抗凋亡分子，与凋亡抑制蛋白或 B 细胞淋巴瘤 2 不同。CIAP 最初被鉴定为一种赋予对生长因子饥饿诱导的细胞凋亡抗性的分子。然而，它在血吸虫中的特征仍不明确。在这里，我们从日本血吸虫中分子鉴定了一种新型的细胞因子诱导的凋亡抑制剂 (SjCIAP)。转录的 SjCIAP 发生在所有研究的发育阶段，包括卵、尾蚴、毛蚴和成虫血吸虫。功能测定表明，SjCIAP 可以抑制人细胞系或血吸虫裂解物中的半胱天冬酶活性。我们的初步结果表明，SjCIAP 可能通过调节凋亡在寄生虫的生活和发育中发挥重要作用，并且 SjCIAP 的药物靶点可能是控制血吸虫病的潜在目标。
