Rao Praveen, Segal Benjamin M
Department of Neurology, University of Michigan Multiple Sclerosis Center and Holtom-Garrett Program in Neuroimmunology, University of Michigan, Ann Arbor, MI, USA.
Methods Mol Biol. 2012;900:363-80. doi: 10.1007/978-1-60761-720-4_18.
Experimental autoimmune encephalomyelitis (EAE) is an inflammatory demyelinating disease of the central nervous system that is induced in laboratory animals by the generation of an immune response against myelin epitopes. It has been used as a prototype of Th1- and/or Th17-driven, organ-specific autoimmunity and as a model for the human disease, multiple sclerosis. In this chapter we describe two classic protocols for EAE induction (active immunization and adoptive transfer of Th1- or Th17-polarized cells) in Subheadings 3.1 and 3.2, respectively. Subheading 3.3 describes methods for rating clinical disease in symptomatic animals. Subheading 3.4 includes instructions for the isolation of mononuclear cells from the inflamed spinal cords of mice with EAE. Subheading 3.5 describes a method for performing the enzyme-linked immunospot assay.
实验性自身免疫性脑脊髓炎（EAE）是一种中枢神经系统的炎性脱髓鞘疾病，通过针对髓鞘表位产生免疫反应在实验动物中诱发。它已被用作Th1和/或Th17驱动的器官特异性自身免疫的原型以及人类疾病多发性硬化症的模型。在本章中，我们分别在3.1和3.2小节中描述了两种诱导EAE的经典方案（主动免疫和Th1或Th17极化细胞的过继转移）。3.3小节描述了对出现症状的动物的临床疾病进行评分的方法。3.4小节包括从患有EAE的小鼠发炎脊髓中分离单核细胞的说明。3.5小节描述了进行酶联免疫斑点测定的方法。
