Applied Genomics Department, Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Brunszvik 2, Martonvásár, Hungary.
Funct Integr Genomics. 2012 Aug;12(3):439-46. doi: 10.1007/s10142-012-0294-z. Epub 2012 Aug 30.
Epitope databases and the protein sequences of published plant genomes are suitable to identify some of the proteins causing food allergies and sensitivities. Brachypodium distachyon, a diploid wild grass with a sequenced genome and low prolamin content, is the closest relative of the allergen cereals, such as wheat or barley. Using the Brachypodium genome sequence, a workflow has been developed to identify potentially harmful proteins which may cause either celiac disease or wheat allergy-related symptoms. Seed tissue-specific expression of the potential allergens has been determined, and intact epitopes following an in silico digestion with several endopeptidases have been identified. Molecular function of allergen proteins has been evaluated using Gene Ontology terms. Biologically overrepresented proteins and potentially allergen protein families have been identified.
表位数据库和已发表的植物基因组的蛋白质序列适合识别一些引起食物过敏和敏感的蛋白质。Brachypodium distachyon 是一种具有测序基因组和低醇溶蛋白含量的二倍体野生草，是过敏原谷物（如小麦或大麦）的近亲。利用 Brachypodium 基因组序列，开发了一种工作流程来识别可能导致乳糜泻或与小麦过敏相关症状的潜在有害蛋白质。已经确定了潜在过敏原的种子组织特异性表达，并通过几种内肽酶的计算机消化鉴定了完整的表位。使用基因本体术语评估了过敏原蛋白的分子功能。已经鉴定了具有生物学意义的过表达蛋白和潜在的过敏原蛋白家族。
