鉴定智利中部入侵物种加利福尼亚罂粟（罂粟科）的新型微卫星标记。
Characterization of novel microsatellite markers for Eschscholzia californica (Papaveraceae), an invasive species in central Chile.
机构信息
Departamento de Ciencias Ecológicas and Instituto de Ecología y Biodiversidad (IEB), Universidad de Chile, Casilla 653, Ñuñoa, Santiago, Chile.
出版信息
Am J Bot. 2012 Sep;99(9):e366-8. doi: 10.3732/ajb.1200076. Epub 2012 Aug 29.
PREMISE OF THE STUDY
We isolated and characterized microsatellite markers for the California poppy, Eschscholzia californica, which is an invasive species in central Chile.
METHODS AND RESULTS
A total of eight polymorphic and six monomorphic loci were developed for the species. Between one and 12 alleles were observed per locus. Polymorphic loci showed heterozygosity ranging from 0 to 0.875 in a sample of 96 individuals obtained from four populations. Only one locus showed significant departures from Hardy-Weinberg equilibrium at all sites.
CONCLUSIONS
The successful microsatellite amplification makes this set of primers an important tool for understanding the recent and future patterns of invasion and adaptation of E. californica into the new Chilean geographic area. The variation detected is currently being used in a more inclusive study that assesses population expansion in central Chile.
研究前提
我们分离并鉴定了加利福尼亚罂粟（Eschscholzia californica）的微卫星标记，加利福尼亚罂粟是智利中部的入侵物种。
方法和结果
为该物种开发了总共 8 个多态性和 6 个单态性位点。每个位点观察到 1 到 12 个等位基因。在从四个种群中获得的 96 个个体的样本中，多态性位点的杂合度范围为 0 到 0.875。只有一个位点在所有地点都显示出与哈迪-温伯格平衡的显著偏离。
结论
成功的微卫星扩增使这组引物成为了解 E. californica 最近和未来入侵和适应新智利地理区域模式的重要工具。目前正在使用检测到的变异进行更具包容性的研究，以评估智利中部的种群扩张。
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