Department of Ecology and Evolutionary Biology, University of Connecticut, Storrs, CT 06269, USA.
Am J Bot. 2012 May;99(5):e220-2. doi: 10.3732/ajb.1100530. Epub 2012 Apr 28.
Microsatellite markers were isolated and characterized in Berberis thunbergii, an invasive and ornamental shrub in the eastern United States, to assess genetic diversity among populations and potentially identify horticultural cultivars.
A total of 12 loci were identified for the species. Eight of the loci were polymorphic and were screened in 24 individuals from two native (Tochigi and Ibaraki prefectures, Japan) and one invasive (Connecticut, USA) population and 21 horticultural cultivars. The number of alleles per locus ranged from three to seven, and observed heterozygosity ranged from 0.048 to 0.636.
These new markers will provide tools for examining genetic relatedness of B. thunbergii plants in the native and invasive range, including phylogeographic studies and assessment of rapid evolution in the invasive range. These markers may also provide tools for examining hybridization with other related species in the invasive range.
本研究从美国东部的入侵性观赏灌木十大功劳中分离和鉴定了微卫星标记，以评估种群间的遗传多样性，并可能鉴定园艺品种。
共鉴定出 12 个物种的位点。其中 8 个位点多态性，在来自两个本地（日本枥木县和茨城县）和一个入侵（美国康涅狄格州）种群的 24 个个体和 21 个园艺品种中进行了筛选。每个位点的等位基因数从 3 到 7 不等，观察到的杂合度从 0.048 到 0.636 不等。
这些新标记将为研究本地和入侵范围内十大功劳植物的遗传相关性提供工具，包括系统地理研究和评估入侵范围内的快速进化。这些标记也可能为研究入侵范围内与其他相关物种的杂交提供工具。
