Ibrahim Khadiga S, Amer Nagat M, El-dossuky Elsaid A, Emara Ahmed M, El-Fattah Abd El-Samei M Abd, Shahy Eman Mohamed
Environmental and Occupational Medicine Department, National Research Centre, Cairo, Egypt.
Industrial Medicine Department, Cairo University, Cairo, Egypt.
Toxicol Ind Health. 2014 Jun;30(5):467-74. doi: 10.1177/0748233712458141. Epub 2012 Aug 29.
Human exposure to benzene in work environment is a global occupational health problem. It is established that benzene requires to be metabolized to induce its effects. Benzene has been associated with various hematotoxins and carcinogens. The aim of this study was to investigate the effect of benzene on complete blood picture, with emphasis of trans, trans-muconic acid (t,t-MA) as a biomarker of benzene in urine, considering the influence of cigarette smoke. A total of 81 workers (61 males and 20 females) have been occupationally exposed to benzene. In addition, 83 workers (55males and 28 females) were also recruited as a control group. Complete blood picture was analyzed and urinary t,t-MA was determined by liquid chromatography. In addition, creatinine in the urine samples was determined. Levels of blood elements (white blood cells, red blood cells and platelets) were decreased among exposed workers compared with the controls. The urinary level of t,t-MA/creatinine of the exposed workers was elevated especially in the smoking group compared to the controls. This study recommends that complete blood picture and t,t-MA are helpful biomarker tests that should be done to detect the early effects of benzene exposure.
人类在工作环境中接触苯是一个全球性的职业健康问题。已证实苯需要经过代谢才能产生其效应。苯与多种血液毒素和致癌物有关。本研究的目的是调查苯对全血细胞图像的影响，重点关注反式、反式粘康酸（t,t-MA）作为尿液中苯的生物标志物，并考虑吸烟的影响。共有81名工人（61名男性和20名女性）职业性接触苯。此外，还招募了83名工人（55名男性和28名女性）作为对照组。分析全血细胞图像，并通过液相色谱法测定尿液中的t,t-MA。此外，还测定了尿液样本中的肌酐。与对照组相比，接触苯的工人血液中的血细胞（白细胞、红细胞和血小板）水平降低。与对照组相比，接触苯的工人尿液中t,t-MA/肌酐水平升高，尤其是在吸烟组。本研究建议全血细胞图像和t,t-MA是有助于检测苯暴露早期效应的生物标志物检测方法。
