Microbiology Division, INIBIC-Complejo Hospitalario Universitario A Coruña, A Coruña, Spain.
J Clin Microbiol. 2012 Nov;50(11):3609-13. doi: 10.1128/JCM.01675-12. Epub 2012 Aug 29.
Infections caused by multidrug-resistant Acinetobacter baumannii constitute a major life-threatening problem worldwide, and early adequate antibiotic therapy is decisive for success. For these reasons, rapid detection of antibiotic susceptibility in this pathogen is a clinical challenge. Two variants of the Micromax kit were evaluated for a rapid detection in situ of susceptibility or resistance to meropenem or ciprofloxacin, separately, in 322 clinical isolates. Release of the nucleoid is the criterion of susceptibility to the beta-lactams (carbapenems), whereas diffusion of DNA fragments emerging from the nucleoid characterizes the quinolone activity. All the susceptible and resistant strains were correctly categorized in 100 min according to the MIC results and CLSI criteria. Thus, our technology is a promising tool for rapid identification of carbapenem and quinolone resistance of A. baumannii strains in hospital settings.
由耐多药鲍曼不动杆菌引起的感染构成了全球范围内的一个主要生命威胁问题，早期充分的抗生素治疗对于成功至关重要。出于这些原因，快速检测这种病原体对抗生素的敏感性是临床面临的挑战。评估了两种 Micromax 试剂盒，用于分别快速检测 322 株临床分离株中对美罗培南或环丙沙星的敏感性或耐药性。核质体的释放是对β-内酰胺类（碳青霉烯类）药物敏感性的标准，而核质体中出现的 DNA 片段的扩散则表征了喹诺酮类药物的活性。根据 MIC 结果和 CLSI 标准，所有敏感和耐药菌株在 100 分钟内都被正确分类。因此，我们的技术是一种有前途的工具，可用于快速鉴定医院环境中鲍曼不动杆菌菌株对碳青霉烯类和喹诺酮类药物的耐药性。
