Institute of Pathology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.
Radiol Oncol. 2011 Mar;45(1):40-5. doi: 10.2478/v10019-011-0002-y. Epub 2011 Feb 23.
Image cytometry can measure numerous nuclear features which could be considered a surrogate end-point marker of molecular genetic changes in a nucleus. The aim of the study was to analyze image cytometric nuclear features in paired samples of primary tumor and neck metastasis in patients with inoperable carcinoma of the head and neck. MATERIALS AND METHODS.: Image cytometric analysis of cell suspensions prepared from primary tumor tissue and fine needle aspiration biopsy cell samples of neck metastases from 21 patients treated with concomitant radiochemotherapy was performed. Nuclear features were correlated with clinical characteristics and response to therapy.
Manifestation of distant metastases and new primaries was associated (p<0.05) with several chromatin characteristics from primary tumor cells, whereas the origin of index cancer and disease response in the neck was related to those in the cells from metastases. Many nuclear features of primary tumors and metastases correlated with the TNM stage.
A specific pattern of correlation between well-established prognostic indicators and nuclear features of samples from primary tumors and those from neck metastases was observed. Image cytometric nuclear features represent a promising candidate marker for recognition of biologically different tumor subgroups.
图像细胞术可以测量许多核特征，这些特征可以被视为细胞核中分子遗传变化的替代终点标记物。本研究的目的是分析无法手术的头颈部癌患者的原发肿瘤和颈部转移灶配对样本中的图像细胞术核特征。
对 21 例接受同期放化疗的患者的原发肿瘤组织和颈部转移灶细针抽吸活检细胞样本制备的细胞悬浮液进行图像细胞术分析。核特征与临床特征和治疗反应相关。
远处转移和新原发灶的表现与原发肿瘤细胞的几种染色质特征相关（p<0.05），而指数癌的起源和颈部的疾病反应与转移灶的细胞相关。许多原发肿瘤和转移灶的核特征与 TNM 分期相关。
观察到明确的预后指标与原发肿瘤和颈部转移灶样本的核特征之间存在特定的相关性模式。图像细胞术核特征是识别生物学上不同肿瘤亚群的有前途的候选标志物。
