Rode Matjaz, Flezar Margareta Strojan, Kogoj-Rode Mirela, Us-Krasovec Marija
Department of Clinical Oral Pathology, University Medical Centre, Slovenia.
Anal Quant Cytol Histol. 2006 Oct;28(5):262-8.
To analyze image cytometric chromatin changes reflected in nuclear texture features and DNA ploidy of oral lichen planus in relation to the normal buccal mucosa and buccal mucosa expressing malignancy-associated changes in cancer patients.
Twenty-eight patients with the reticular form of oral lichen planus, with a follow-up period of 25 years, 50 healthy controls and 50 lung cancer patients were included in the study. Scrapings of buccal mucosa were suspended in transport medium. Monolayer filter preparations were Feulgen-thionin stained. Image cytometric analysis was performed by Cyto-Savant.
All oral lichen planus specimens in our study were diploid. In univariate analysis, differences between the normal buccal mucosa and oral lichen planus were found in several nuclear texture features, which gave an 80% correct classification rate in multivariate analysis. In the second part of the study, the classifier that recognizes malignancy-associated changes on the buccal mucosa of patients with lung cancer correctly recognized > 80% of oral lichen planus samples as normal buccal mucosa.
Our results indicate that chromatin changes in oral lichen planus exist compared to normal cells; however, the chromatin structure of the reticular form of oral lichen planus does not express malignancy-associated changes and is more similar to normal squamous cells.
分析口腔扁平苔藓细胞核纹理特征和DNA倍体所反映的图像细胞计量学染色质变化，并与正常颊黏膜以及癌症患者颊黏膜中表达恶性相关变化的情况进行比较。
本研究纳入了28例网状型口腔扁平苔藓患者，随访期为25年，以及50名健康对照者和50名肺癌患者。颊黏膜刮片悬浮于转运培养基中。单层滤膜制剂采用Feulgen硫堇染色。通过Cyto-Savant进行图像细胞计量分析。
本研究中所有口腔扁平苔藓标本均为二倍体。在单变量分析中，正常颊黏膜与口腔扁平苔藓在几个核纹理特征上存在差异，多变量分析的正确分类率为80%。在研究的第二部分，识别肺癌患者颊黏膜恶性相关变化的分类器将>80%的口腔扁平苔藓样本正确识别为正常颊黏膜。
我们的结果表明，与正常细胞相比，口腔扁平苔藓存在染色质变化；然而，网状型口腔扁平苔藓的染色质结构未表达恶性相关变化，且更类似于正常鳞状细胞。
