Department of Urology; Centre Hospitalier Universitaire Vaudois; Lausanne, Switzerland.
Oncoimmunology. 2012 Aug 1;1(5):694-698. doi: 10.4161/onci.20526.
To gain further insights into the role of T lymphocytes in immune responses against bladder tumors, we developed a method that monitors the presence of functional antigen-specific T cells in the urine of non-muscle invasive bladder cancer patients. As relatively few immune cells can usually be recovered from urine, we examined different isolation/amplification protocols and took advantage of patients treated with weekly intravesical instillations of Bacillus Calmette-Guérin, resulting in large amounts of immune cells into urine. Our findings demonstrate that, upon in vitro amplification, antigen-specific T cells can be detected by an interferon γ (IFNγ)-specific ELISPOT assay.
为了深入了解 T 淋巴细胞在针对膀胱癌的免疫反应中的作用，我们开发了一种方法，可以监测非肌肉浸润性膀胱癌患者尿液中功能性抗原特异性 T 细胞的存在。由于通常从尿液中可以回收的免疫细胞数量相对较少，因此我们检查了不同的分离/扩增方案，并利用每周膀胱内灌注卡介苗治疗的患者，使大量免疫细胞进入尿液。我们的研究结果表明，经过体外扩增，可以通过干扰素 γ（IFNγ）特异性 ELISPOT 测定法检测到抗原特异性 T 细胞。
