Li Li, Zhang Yan, Pu Jianzhang, Li Guifang, Ye Ming
Department of Otolaryngology, Beijing Shunyi Hospital, Beijing, 101300, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2012 Jun;26(11):502-6.
To study the expression of PCNA and LNGFr in olfactory epithelium of patients suffering from dysosmia caused by chronic sinusitis, and the function of LNGFr.
Forty-six patients undergoing FESS were chosen. Before operation, their olfactory functions were examined with CCCRC. According to their CCCRC scores, they were divided into three groups. Group A: Patients with chronic sinusitis and dysosmia 25 cases; Group B: Patients with chronic sinusitis and a normal olfactory function 10 cases; Group C: Patients with deviation of nasal septum and a normal olfactory function 11 cases. The expressions of PCNA and LNGFr were measured in olfactory mucosas of the three groups by immunohistochemistry.
In basal cells, the expression of PCNA and LNGFr in group A was higher than that in group B (P < 0.01). and in group C (P < 0.01). There was negative correlation between positive cells of PCNA and CCCRC score in basal cells of group A (r = -0.7441, P < 0.01); There was negative correlation between integral optical density of LNGFr and CCCRC score in basal cells of group A (r = -0.4407, P < 0.05). There was positive correlation between positive cells of PCNA and integral optical density of LNGFr in basal cells of group A (r = 0.5317, P < 0.01).
Basal cells proliferated dramatically in patients suffering from dysosmia caused by chronic sinusitis. The proliferating capacity of basal cells was related to up-regulation of LNGFr expression.
研究慢性鼻窦炎所致嗅觉障碍患者嗅上皮中增殖细胞核抗原（PCNA）和低亲和力神经生长因子受体（LNGFr）的表达情况以及LNGFr的功能。
选取46例行功能性鼻内镜鼻窦手术（FESS）的患者。术前采用嗅觉障碍临床研究中心（CCCRC）评分法检查其嗅觉功能。根据CCCRC评分将患者分为三组。A组：慢性鼻窦炎伴嗅觉障碍患者25例；B组：慢性鼻窦炎伴嗅觉功能正常患者10例；C组：鼻中隔偏曲伴嗅觉功能正常患者11例。采用免疫组织化学法检测三组患者嗅黏膜中PCNA和LNGFr的表达。
在基底细胞中，A组PCNA和LNGFr的表达高于B组（P<0.01），也高于C组（P<0.01）。A组基底细胞中PCNA阳性细胞数与CCCRC评分呈负相关（r=-0.7441，P<0.01）；A组基底细胞中LNGFr积分光密度与CCCRC评分呈负相关（r=-0.4407，P<0.05）。A组基底细胞中PCNA阳性细胞数与LNGFr积分光密度呈正相关（r=0.5317，P<0.01）。
慢性鼻窦炎所致嗅觉障碍患者的基底细胞显著增殖。基底细胞的增殖能力与LNGFr表达上调有关。
