Universidad Nacional de Tucuman, San Miguel de Tucuman, Argentina.
Mol Plant Microbe Interact. 2012 Nov;25(11):1430-9. doi: 10.1094/MPMI-12-11-0306.
In an incompatible interaction between Colletotrichum fragariae and strawberry plants, the accumulation of phenolic compounds in plant leaves was observed. A particularly abundant penta-esterified ellagitannin that accumulated in response to pathogen attack was identified as 1-0-galloyl-2,3;4,6-bis-hexahydroxydiphenoyl-β-d-glucopyranose (HeT) by mass spectroscopy and nuclear magnetic resonance. Foliar application of purified HeT prior to inoculation with a virulent pathogen was shown to increase resistance toward C. acutatum in strawberry plants and to Xanthomonas citri subsp. citri in lemon plants. The induced resistance in strawberry was associated with a rapid oxidative burst, callose deposition, a transient increase of salicylic acid in phloem, and induction of gene expression responsive to salicylic acid. Results obtained suggested that HeT could be a common plant defense response molecule capable of inducing pathogen resistance in different plant species.
在草莓病原菌 Colletotrichum fragariae 和草莓植株的不相容互作中，观察到植物叶片中酚类化合物的积累。通过质谱和核磁共振鉴定，发现一种特别丰富的五酯基鞣花单宁，它是对病原体攻击的响应而积累的，被鉴定为 1-0-没食子酰基-2,3;4,6-二己酰基-β-d-葡萄糖吡喃糖苷（HeT）。在接种强毒病原菌之前，将纯化的 HeT 施用于叶片，可提高草莓植株对 C. acutatum 的抗性和柠檬植株对柑橘黄单胞菌的抗性。草莓的诱导抗性与快速氧化爆发、胼胝质沉积、韧皮部水杨酸的短暂增加以及对水杨酸反应的基因表达诱导有关。研究结果表明，HeT 可能是一种常见的植物防御反应分子，能够诱导不同植物物种的抗病性。
