Research and Innovation Centre, Edmund Mach Foundation, San Michele all'Adige, Italy.
J Agric Food Chem. 2012 Mar 14;60(10):2507-16. doi: 10.1021/jf2052256. Epub 2012 Mar 1.
Although the composition of strawberry fruit has been extensively studied, especially for the most abundant phenolic compounds, agrimoniin has never been univocally identified as one of the most abundant phenolic compounds in the fruit. In this study agrimoniin was isolated in the fruit of Fragaria vesca and its structure characterized. Furthermore, its presence was definitively established to be the main ellagitannin in both F. vesca and Fragaria ananassa D. fruit. The presence of sanguiin H-6 and lambertianin C as minor compounds was confirmed in both F. vesca and F. ananassa D. samples. For the first time here is reported the full NMR assignments for agrimoniin. These data should represent a point of reference for NMR analysis of this and other structurally related ellagitannins. Finally, the establishment of an HPLC protocol for separation provided information making it possible to avoid confusion with sanguiin H-6, the main ellagitannin in Rubus species, which is also present in strawberries but at a much lower concentration.
尽管草莓果实的成分已经得到了广泛的研究，特别是对于最丰富的酚类化合物，但 agrimoniin 从未被明确鉴定为果实中最丰富的酚类化合物之一。在这项研究中，agrimoniin 在悬钩子果实中被分离出来，并对其结构进行了表征。此外，它的存在被确定为 F. vesca 和 Fragaria ananassa D.果实中主要的鞣花单宁。在 F. vesca 和 F. ananassa D. 样品中均确认存在 sanguiin H-6 和 lambertianin C 等少量化合物。本文首次报道了 agrimoniin 的完整 NMR 分配。这些数据应该为该化合物和其他结构相关的鞣花单宁的 NMR 分析提供参考。最后，建立了 HPLC 分离方案，提供了信息，避免了与桑色素 H-6（悬钩子属植物中的主要鞣花单宁）混淆，桑色素 H-6 也存在于草莓中，但浓度要低得多。
