Donover Preston S, Yohn Marlin, Sim Matthew, Wright Andrew, Gowda Sandesh, Allee Chip, Schabdach Amanda R, Reichman Melvin
Lankenau Institute for Medical Research Chemical Genomics Center-LCGC, Inc., 100 Lancaster Avenue, Wynnewood, PA 19096, USA.
Comb Chem High Throughput Screen. 2013 Mar;16(3):180-8. doi: 10.2174/1386207311316030003.
The Lankenau Institute for Medical Research Chemical Genomics Center, Inc. has developed a new (patents issued and pending) Nanotube Automated Repository System (NARS) for dynamic storage of millions of 'single-shot' samples stored in a new monolithic microtiter-storage tube plate of our own design we call 'nanotubes.' We have integrated the NARS with customized software to efficiently access up to 10,000,000 samples stored continuously frozen (-20°C) in a dehumidified enclosure and sealed in a new microtiter NARS plate that is SBS compliant. Additional software was developed to analyze HTS data from orthogonally pooled compound libraries. Following 'de-convolution' of pooled HTS data, the software designates confirmatory retest samples to be 'cherry-picked' using the NARS. The application of a new, fully-integrated infrastructure for new leads discovery is described in detail. Other applications for our technologies and new infrastructure are discussed.
兰肯瑙医学研究所化学基因组学中心有限公司开发了一种新的（已获专利及正在申请专利）纳米管自动存储系统（NARS），用于动态存储数百万个“单次使用”样本，这些样本存储在我们自行设计的新型整体式微量滴定存储管板中，我们将其称为“纳米管”。我们已将NARS与定制软件集成，以便高效访问连续保存在-20°C冷冻状态、位于除湿箱内且密封在符合SBS标准的新型微量滴定NARS板中的多达1000万个样本。还开发了额外的软件来分析来自正交汇集化合物文库的高通量筛选（HTS）数据。在对汇集的HTS数据进行“反卷积”之后，该软件指定使用NARS“挑选”确认性复测样本。详细描述了一种用于发现新先导物的全新完全集成基础设施的应用。还讨论了我们的技术和新基础设施的其他应用。
