通过高通量筛选（HTS）加速新线索发现的新型信息学和自动化基础设施。
New informatics and automated infrastructure to accelerate new leads discovery by high throughput screening (HTS).
作者信息
Donover Preston S, Yohn Marlin, Sim Matthew, Wright Andrew, Gowda Sandesh, Allee Chip, Schabdach Amanda R, Reichman Melvin
机构信息
Lankenau Institute for Medical Research Chemical Genomics Center-LCGC, Inc., 100 Lancaster Avenue, Wynnewood, PA 19096, USA.
出版信息
Comb Chem High Throughput Screen. 2013 Mar;16(3):180-8. doi: 10.2174/1386207311316030003.
The Lankenau Institute for Medical Research Chemical Genomics Center, Inc. has developed a new (patents issued and pending) Nanotube Automated Repository System (NARS) for dynamic storage of millions of 'single-shot' samples stored in a new monolithic microtiter-storage tube plate of our own design we call 'nanotubes.' We have integrated the NARS with customized software to efficiently access up to 10,000,000 samples stored continuously frozen (-20°C) in a dehumidified enclosure and sealed in a new microtiter NARS plate that is SBS compliant. Additional software was developed to analyze HTS data from orthogonally pooled compound libraries. Following 'de-convolution' of pooled HTS data, the software designates confirmatory retest samples to be 'cherry-picked' using the NARS. The application of a new, fully-integrated infrastructure for new leads discovery is described in detail. Other applications for our technologies and new infrastructure are discussed.
兰肯瑙医学研究所化学基因组学中心有限公司开发了一种新的（已获专利及正在申请专利）纳米管自动存储系统（NARS），用于动态存储数百万个“单次使用”样本，这些样本存储在我们自行设计的新型整体式微量滴定存储管板中，我们将其称为“纳米管”。我们已将NARS与定制软件集成，以便高效访问连续保存在-20°C冷冻状态、位于除湿箱内且密封在符合SBS标准的新型微量滴定NARS板中的多达1000万个样本。还开发了额外的软件来分析来自正交汇集化合物文库的高通量筛选（HTS）数据。在对汇集的HTS数据进行“反卷积”之后，该软件指定使用NARS“挑选”确认性复测样本。详细描述了一种用于发现新先导物的全新完全集成基础设施的应用。还讨论了我们的技术和新基础设施的其他应用。
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