Department of Chemistry, Washington University in St. Louis, Missouri 63130, USA.
Langmuir. 2012 Sep 25;28(38):13652-9. doi: 10.1021/la300670a. Epub 2012 Sep 17.
Patterned supported lipid bilayers (SLBs) provide a model system for studying fluid lipid bilayers and transmembrane proteins in an array format. SLB arrays self-assemble on patterned self-assembled monolayers (SAMs) consisting of hexadecanethiol and glycol-terminated regions. While the mechanism of SLB formation on glass has been studied extensively, the formation of SLBs on other substrates is not necessarily well understood. Moreover, SLB arrays on patterned SAMs represent an intriguing system, since lipid vesicles do not adhere to glycol-terminated monolayers. Here, we utilize surface plasmon resonance imaging (SPRi) and kinetic analysis to examine the mechanism of SLB formation on the glycol-terminated regions of patterned SAMs and supported lipid monolayer (SLM) formation on alkyl-terminated regions of patterned SAMs. We determine that vesicles rupture to form a patterned SLB through a two-step mechanism that is dependent upon vesicle attachment at the interface of the two regions of the patterned monolayer.
图案化支撑脂质双层（SLB）为研究在阵列格式中的流体脂质双层和跨膜蛋白提供了模型系统。SLB 阵列自组装在由十六烷硫醇和乙二醇末端区域组成的图案化自组装单层（SAM）上。虽然已经对玻璃上的 SLB 形成机制进行了广泛研究，但其他基底上的 SLB 形成机制不一定很清楚。此外，图案化 SAM 上的 SLB 阵列代表了一个有趣的系统，因为脂质囊泡不会附着在乙二醇末端单层上。在这里，我们利用表面等离子体共振成像（SPRi）和动力学分析来研究在图案化 SAM 的乙二醇末端区域上形成 SLB 的机制，以及在图案化 SAM 的烷基末端区域上形成支撑脂质单层（SLM）的机制。我们确定囊泡通过依赖于在图案化单层的两个区域的界面处的囊泡附着的两步机制破裂以形成图案化 SLB。
