Department of Chemistry and Center for Materials Innovation, Washington University in St. Louis, St. Louis, Missouri 63130, USA.
Langmuir. 2011 Oct 4;27(19):12052-7. doi: 10.1021/la201839w. Epub 2011 Sep 9.
Supported lipid bilayers (SLBs) formed on many different substrates have been widely used in the study of lipid bilayers. However, most SLBs suffer from inhomogeneities due to interactions between the lipid bilayer and the substrate. In order to avoid this problem, we have used microcontact printing to create patterned SLBs on top of ethylene-glycol-terminated self-assembled monolayers (SAMs). Glycol-terminated SAMs have previously been shown to resist absorbance of biomolecules including lipid vesicles. In our system, patterned lipid bilayer regions are separated by lipid monolayers, which form over the patterned hexadecanethiol portions of the surface. Furthermore, we demonstrate that α-hemolysin, a large transmembrane protein, inserts preferentially into the lipid bilayer regions of the substrate.
支持的脂质双层（SLB）在许多不同的基质上形成，被广泛用于脂质双层的研究。然而，由于脂质双层与基质之间的相互作用，大多数 SLB 存在不均匀性。为了避免这个问题，我们使用微接触印刷术在乙二醇封端的自组装单层（SAM）上创建图案化的 SLB。之前已经表明，乙二醇封端的 SAM 可以抵抗包括脂质囊泡在内的生物分子的吸收。在我们的系统中，图案化的脂质双层区域由脂质单层隔开，脂质单层在表面的图案化十六硫醇部分上形成。此外，我们证明了一种大的跨膜蛋白α-溶血素优先插入到基底的脂质双层区域。
