van Tilburg A J, de Rooij F W, van Blankenstein M, van den Berg J W, Bosman-Jacobs E P
Department of Internal Medicine II, University Hospital Dijkzigt, Rotterdam, The Netherlands.
Gastroenterology. 1990 Jan;98(1):25-32. doi: 10.1016/0016-5085(90)91286-f.
Ileal Na+-dependent bile acid transport was quantified in vitro as the uptake of 3H-taurocholate into brush-border membrane vesicles. Vesicles were prepared from ileal biopsies of 158 patients placed in 10 diagnostic categories. Active bile acid transport (expressed as picomoles taurocholate uptake per milligram brush-border membrane protein per 15 s, median and interquartile ranges indicated) did not differ significantly in 6 categories: irritable bowel syndrome (71, 35-97; n = 21), colon polyps (42, 30-89; n = 29), colitis (62, 33-91; n = 31), postvagotomy or postcholecystectomy (69, 37-97; n = 11), diarrhea without increased bile acid loss (58, 48-85; n = 12), and lack of gastrointestinal pathology (74, 45-103; n = 22). A decreased active bile acid transport was found in 3 categories: ileal disease (4, 1-36; n = 11), partial ileal resection (5, 1-35; n = 5), and constipation (41, 22-50; n = 8). Bile acid transport was increased in patients with bile acid-losing diarrhea with endoscopically and histologically normal ilea (111, 94-135; n = 8). These findings indicate that a low fecal bile acid loss, presumed to be present in constipated patients, is associated with a low Na+-dependent ileal bile acid transport and a high bile acid loss is associated with a high active bile acid transport. Ileal bile acid transport might be regulated by the availability of bile acids to the ileal enterocytes.
回肠钠依赖性胆汁酸转运在体外通过测量³H-牛磺胆酸盐摄取到刷状缘膜囊泡中的量来进行定量。囊泡取自158例患者的回肠活检组织，这些患者分属10个诊断类别。在6个类别中，活性胆汁酸转运（表示为每15秒每毫克刷状缘膜蛋白摄取牛磺胆酸盐的皮摩尔数，给出中位数和四分位数间距）无显著差异：肠易激综合征（71，35 - 97；n = 21）、结肠息肉（42，30 - 89；n = 29）、结肠炎（62，33 - 91；n = 31）、迷走神经切断术或胆囊切除术后（69，37 - 97；n = 11）、无胆汁酸丢失增加的腹泻（58，48 - 85；n = 12）以及无胃肠道病变（74，45 - 103；n = 22）。在3个类别中发现活性胆汁酸转运降低：回肠疾病（4，1 - 36；n = 11）、部分回肠切除术（5，1 - 35；n = 5）和便秘（41，22 - 50；n = 8）。在内镜和组织学检查显示回肠正常的胆汁酸丢失性腹泻患者中，胆汁酸转运增加（出111，94 - 135；n = 8）。这些发现表明，推测便秘患者粪便胆汁酸丢失较低，这与回肠钠依赖性胆汁酸转运较低相关，而胆汁酸丢失较高则与活性胆汁酸转运较高相关。回肠胆汁酸转运可能受胆汁酸向回肠肠上皮细胞的可利用性调节。
