de Rooij F W, van den Berg J W, Sinaasappel M, Bosman-Jacobs E P, Touw-Blommesteijn A C
Acta Paediatr Scand Suppl. 1985;317:28-30. doi: 10.1111/j.1651-2227.1985.tb14931.x.
Increased fecal bile acid loss in cystic fibrosis (CF) may result from ileal dysfunction. A method to quantitate in vitro Na+-dependent taurocholate uptake into brush border membrane vesicles prepared from frozen ileum and ileal biopsy specimen is described. This transport across the ileal brush border membrane can be measured selectively, in contrast to in vivo measurements which represent a complex overall process. Preliminary results obtained with ileal specimen of 2 CF patients, suggest that in vitro bile acid uptake is low but not abnormal.
