Department of Basic Medical Sciences, Section of Human Anatomy, University of Bari, Piazza Giulio Cesare 1, I-70124 Bari, Italy.
Pathology. 2012 Oct;44(6):557-61. doi: 10.1097/PAT.0b013e3283580388.
To study the importance of IκBα in NF-κB signal transduction, we analysed the IκBα expression in monocytes from Sjögren's syndrome (SS) patients versus healthy controls.
Monocytes were obtained from the peripheral blood of 30 SS patients and 23 healthy subjects. IκBα expression was studied by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR), real-time PCR, immunoblotting, flow cytometry and enzyme linked immunosorbent assay (ELISA).
Analysis of the gene and protein expression profiles of SS monocytes revealed a down-regulation of IκBα, and in all the Sjögren's syndrome cases examined, serum IκBα levels were significantly decreased in comparison with controls.
Our findings clearly demonstrate changes in the levels of IκBα in SS monocytes, suggesting that the attenuated expression of IκBα could contribute to the deregulation of NF-κB pathways in the SS pathogenesis. Decreased expression of IκBα may specifically amplify cytokines production and inflammatory response linked to Sjögren's syndrome.
为了研究 IκBα 在 NF-κB 信号转导中的重要性，我们分析了干燥综合征（SS）患者和健康对照者单核细胞中 IκBα 的表达。
从 30 例 SS 患者和 23 例健康受试者的外周血中获得单核细胞。通过半定量逆转录聚合酶链反应（RT-PCR）、实时 PCR、免疫印迹、流式细胞术和酶联免疫吸附试验（ELISA）研究 IκBα 的表达。
对 SS 单核细胞的基因和蛋白表达谱分析显示 IκBα 下调，在所有检查的干燥综合征病例中，与对照组相比，血清 IκBα 水平显著降低。
我们的研究结果清楚地表明 SS 单核细胞中 IκBα 水平的变化，表明 IκBα 表达减弱可能导致 SS 发病机制中 NF-κB 途径的失调。IκBα 的表达降低可能特异性地放大与干燥综合征相关的细胞因子产生和炎症反应。
