Department of Rheumatology and Clinical Immunology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China.
Xiamen Key Laboratory of Rheumatology and Clinical Immunology, Xiamen Science and Technology Bureau, Xiamen, China.
Front Immunol. 2022 Mar 4;13:864920. doi: 10.3389/fimmu.2022.864920. eCollection 2022.
Recent studies have proven the existence of distinct monocyte subsets, which play a significant role in the development of some rheumatic diseases such as systemic lupus erythematosus (SLE). This study was performed to define the changes of monocyte subsets in patients with Sjögren's Syndrome (SjS).
Single cell RNA-sequencing (scRNA-seq) data of monocytes from SjS patients and controls were analyzed. The transcriptomic changes in monocyte subsets between SjS and controls were identified and potential key functional pathways involved in SjS development were also explored.
A total of 11 monocyte subsets were identified in the scRNA-seq analyses of monocytes. A new monocyte subset characterized by higher expression of VNN2 (GPI-80) and S100A12 (Monocyte cluster 3) was identified, and it was increased in SjS patients. Compared with controls, almost all monocyte subsets from SjS patients had increased expression of TNFSF10 (TRAIL). Moreover, interferon (IFN)-related and neutrophil activation-associated pathways were main up-regulated pathways in the monocytes of SjS patients.
This study uncovered the abnormal changes in monocyte subsets and their transcriptomic changes in SjS patients, and identified TNFSF10 monocytes as a potential key player in SjS pathogenesis and a promising target for SjS treatment.
最近的研究证明了单核细胞亚群的存在，它们在一些风湿性疾病（如系统性红斑狼疮[SLE]）的发展中起着重要作用。本研究旨在确定干燥综合征（SjS）患者单核细胞亚群的变化。
对 SjS 患者和对照者的单核细胞单细胞 RNA 测序（scRNA-seq）数据进行分析。鉴定 SjS 和对照者之间单核细胞亚群的转录组变化，并探讨涉及 SjS 发展的潜在关键功能途径。
在单核细胞 scRNA-seq 分析中鉴定出 11 种单核细胞亚群。鉴定出一个新的单核细胞亚群，其特征是更高表达 VNN2（GPI-80）和 S100A12（单核细胞簇 3），且在 SjS 患者中增加。与对照者相比，SjS 患者的几乎所有单核细胞亚群均表现出更高的 TNFSF10（TRAIL）表达。此外，干扰素（IFN）相关和中性粒细胞激活相关途径是 SjS 患者单核细胞中主要上调的途径。
本研究揭示了 SjS 患者单核细胞亚群及其转录组的异常变化，并确定了 TNFSF10 单核细胞是 SjS 发病机制中的一个潜在关键因素，也是 SjS 治疗的一个有前途的靶点。
