School of Traditional Chinese Medicine, Capital Medical University, Beijing, China.
Chin J Integr Med. 2012 Sep;18(9):670-5. doi: 10.1007/s11655-012-1202-z. Epub 2012 Aug 31.
To investigate the effect of Yisui Shengxue Granule (, YSSXG), a complex Chinese medicine, on the oxidative damage of erythrocytes from patients with hemoglobin H (HbH) disease.
Twenty-two patients with HbH disease and 22 healthy volunteers were observed. YSSXG was given to patients with HbH disease for 3 months. Before and after the 3-month treatment, blood parameters [hemoglobin (Hb), red blood cells (RBCs), and reticulocyte percent (Ret)] were examined; inclusion bodies in erythrocytes were observed by transmission electron microscopy (TEM); activities of antioxidant defense enzymes [superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (Cat)] and erythrocyte membrane malondialdehyde (MDA) concentrations were determined.
In patients with HbH disease, measured values of RBC and Hb obtained from the first to the third months after treatment with YSSXG were significantly higher than before treatment (P<0.01). Measured values of Ret from the second to the third months after treatment were significantly lower than before treatment (P<0.05 and P<0.01, respectively). Prior to treatment with YSSXG, TEM images of RBCs showed the presence of numerous inclusion bodies. After treatment with YSSXG, the amount and volume of inclusion bodies decreased. Treatment with YSSXG also led to a significant increase in SOD activity (P<0.01), a decrease in Cat activity (P<0.01), and no significant differences in GSHPx activity (P>0.05) or MDA concentration (P>0.05). However, compared with the healthy counterparts, SOD, GSH-Px, and Cat activities presented at high levels (P<0.01) both before and after treatment.
YSSXG could improve the degree of hemolysis and anemia in patients with HbH disease. The mechanism may be related to its antioxidative effects, which could elevate the activity of total SOD in erythrocytes and efficiently inhibit the oxidative precipitation of β-globin chains.
探讨益髓生血颗粒（YSSXG）对血红蛋白 H（HbH）病患者红细胞氧化损伤的影响。
观察 22 例 HbH 病患者和 22 名健康志愿者。给予 HbH 病患者 YSSXG 治疗 3 个月。治疗前及治疗 3 个月后检测血参数[血红蛋白（Hb）、红细胞（RBC）和网织红细胞百分率（Ret）]；透射电镜观察红细胞内包涵体；测定抗氧化防御酶[超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（Cat）]活性和红细胞膜丙二醛（MDA）浓度。
HbH 病患者经 YSSXG 治疗 1、2、3 个月后 RBC 和 Hb 检测值均明显高于治疗前（P<0.01）；Ret 检测值于治疗 2、3 个月后均明显低于治疗前（P<0.05 和 P<0.01）。治疗前 RBC 电镜下可见大量包涵体，治疗后包涵体数量和体积减少。YSSXG 治疗还可使 SOD 活性显著升高（P<0.01），Cat 活性显著降低（P<0.01），GSH-Px 活性无显著差异（P>0.05），MDA 浓度无显著差异（P>0.05）。但治疗前后与健康对照者比较，SOD、GSH-Px 和 Cat 活性均较高（P<0.01）。
YSSXG 可改善 HbH 病患者的溶血和贫血程度，其机制可能与抗氧化作用有关，能提高红细胞总 SOD 活性，有效抑制β-珠蛋白链的氧化沉淀。
