Mycological Research Center, Fujian Agricultural and Forestry University, Fuzhou, China.
Genome. 2012 Sep;55(9):667-72. doi: 10.1139/g2012-043. Epub 2012 Sep 1.
The mitochondrial intermediate peptidase (MIP) gene is conserved in fungi. It is linked closely with the mating-type A (mtA) gene. In this study, a fragment of the MIP gene in Volvariella volvacea (Bull. ex Fr.) Singer was first cloned by homologue-based cloning technology. Subsequently, the entire MIP DNA sequence (PYd21-MIP) was obtained after the fragment was compared with the genomic data through BLAST analysis. The PYd21-MIP sequence appeared to be homologous with the MIP gene in other fungi. Phylogenetic analysis of PYd21-MIP and other MIP sequences from diverse fungi agreed with the current organism phylogeny. Analysis of protein domains by InterProScan software and motif searching demonstrated that PYd21-MIP encodes a homologous MIP protein. These data support the hypothesis that the PYd21-MIP protein is a Hog-MIP protein homologue from V. volvacea.
线粒体中间肽酶（MIP）基因在真菌中保守。它与交配型 A（mtA）基因紧密相连。在这项研究中，我们首次通过同源克隆技术克隆了草菇（Volvariella volvacea（Bull. ex Fr.）Singer）中的 MIP 基因片段。随后，通过 BLAST 分析将该片段与基因组数据进行比较，获得了整个 MIP DNA 序列（PYd21-MIP）。PYd21-MIP 序列与其他真菌中的 MIP 基因具有同源性。通过 InterProScan 软件对 PYd21-MIP 和其他来自不同真菌的 MIP 序列进行蛋白质结构域分析和基序搜索表明，PYd21-MIP 编码一个同源的 MIP 蛋白。这些数据支持 PYd21-MIP 蛋白是草菇 Hog-MIP 蛋白同源物的假设。
