Gong Ming, Chen Mingjie, Wang Hong, Zhu Qiuming, Tan Qi
National Engineering Research Center of Edible Fungi, Ministry of Science and Technology; Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, P.R. China; Department of Computer Science, University of Nebraska at Omaha, Omaha, Nebraska 68182.
National Engineering Research Center of Edible Fungi, Ministry of Science and Technology; Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, P.R. China.
Mycologia. 2015 Mar-Apr;107(2):313-8. doi: 10.3852/14-159. Epub 2014 Dec 30.
Cryogenic autolysis is a typical phenomenon of abnormal metabolism in Volvariella volvacea. Recent studies have identified 20 significantly up-regulated genes via high-throughput sequencing of the mRNAs expressed in the mycelia of V. volvacea after cold exposure. Among these significantly up-regulated genes, 15 annotated genes were used for functional annotation cluster analysis. Our results showed that the cyclin-like F-box domain (FBDC) formed the functional cluster with the lowest P-value. We also observed a significant expansion of FBDC families in V. volvacea. Among these, the FBDC3 family displayed the maximal gene expansion in V. volvacea. Gene expression profiling analysis revealed only one FBDC gene in V. volvacea (FBDV1) that was significantly up-regulated, which is located in the FBDC3 family. Comparative genomics analysis revealed the homologous sequences of FBDV1 with high similarity were clustered on the same scaffold. However, FBDV1 was located far from these clusters, indicating the divergence of duplicated genes. Relative time estimation and rate test provided evidence for the divergence of FBDV1 after recent duplications. Real-time RT-PCR analysis confirmed that the expression of the FBDV1 was significantly up-regulated (P < 0.001) after cold-treatment of V. volvacea for 4 h. These observations suggest that the FBDV1 is involved in the cryogenic autolysis of V. volvacea.
低温自溶是草菇异常代谢的典型现象。最近的研究通过对草菇菌丝体冷暴露后表达的mRNA进行高通量测序，鉴定出20个显著上调的基因。在这些显著上调的基因中，15个注释基因用于功能注释聚类分析。我们的结果表明，细胞周期蛋白样F-box结构域（FBDC）形成了P值最低的功能聚类。我们还观察到草菇中FBDC家族有显著扩张。其中，FBDC3家族在草菇中显示出最大的基因扩张。基因表达谱分析显示草菇中只有一个FBDC基因（FBDV1）显著上调，它位于FBDC3家族中。比较基因组学分析表明，FBDV1的高度相似同源序列聚集在同一支架上。然而，FBDV1与这些聚类相距较远，表明重复基因的分化。相对时间估计和速率测试为FBDV1在最近复制后的分化提供了证据。实时RT-PCR分析证实，草菇经4小时冷处理后，FBDV1的表达显著上调（P < 0.001）。这些观察结果表明，FBDV1参与了草菇的低温自溶过程。
