Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands.
J Cosmet Dermatol. 2012 Sep;11(3):213-22. doi: 10.1111/j.1473-2165.2012.00631.x.
Imedeen™ is a cosmeceutical that provides nutrients to the skin. One of its active ingredients is the Marine Complex™ (MC).
The aim of this study was to evaluate whether MC affects skin morphogenesis differently in female and male human skin equivalents (HSEs).
Human skin equivalents were established with cells obtained from female or male donors between 30 and 45 years of age and cultured for seven or 11 weeks in the presence or absence of MC. Using immunohistochemistry, we examined early differentiation by keratin 10 expression, (hyper)proliferation by keratin 17 and Ki67, and basement membrane composition by laminin 332 and collagen type VII. In addition, the expression of collagen type I and the secretion of pro-collagen I were measured.
Marine Complex strongly increased the number of Ki67-positive epidermal cells in female HSEs. In the dermis, MC significantly stimulated the amount of secreted pro-collagen I and increased the deposition of laminin 332 and collagen type VII. Furthermore, MC prolonged the viable phase of HSEs by slowing down its natural degradation. After 11 weeks of culturing, the MC-treated HSEs showed higher numbers of viable epidermal cell layers and a thicker dermal extracellular matrix compared with controls. In contrast, these effects were less pronounced in male HSEs.
The MC nutrient positively stimulated overall HSE tissue formation and prolonged the longevity of both female and male HSEs. The ability of MC to stimulate the deposition of basement membrane and dermal components can be used to combat 2 human skin aging in vivo.
Imedeen™ 是一种提供皮肤营养的美容护肤品。其活性成分之一是海洋复合物（MC）。
本研究旨在评估 MC 是否会对女性和男性人体皮肤等效物（HSE）的皮肤形态发生产生不同的影响。
使用来自 30 至 45 岁女性和男性供体的细胞建立人体皮肤等效物，并在存在或不存在 MC 的情况下培养 7 或 11 周。通过免疫组织化学，我们检查了角蛋白 10 表达的早期分化、角蛋白 17 和 Ki67 表示的（过度）增殖以及层粘连蛋白 332 和 VII 型胶原的基底膜组成。此外，还测量了 I 型胶原的表达和前胶原 I 的分泌。
海洋复合物强烈增加了女性 HSE 中 Ki67 阳性表皮细胞的数量。在真皮中，MC 显著刺激分泌的前胶原 I 的量，并增加层粘连蛋白 332 和 VII 型胶原的沉积。此外，MC 通过减缓其天然降解来延长 HSE 的存活期。经过 11 周的培养，与对照组相比，MC 处理的 HSE 显示出更多的存活表皮细胞层和更厚的真皮细胞外基质。相比之下，这些影响在男性 HSE 中不太明显。
MC 营养素积极刺激整体 HSE 组织形成，并延长女性和男性 HSE 的寿命。MC 刺激基底膜和真皮成分沉积的能力可用于对抗体内 2 种人类皮肤衰老。
