Naveen Kumar D R, Cijo George V, Suresh P K, Ashok Kumar R
School of Biosciences and Technology, VIT University, Vellore, India.
Asian Pac J Cancer Prev. 2012;13(6):2729-34. doi: 10.7314/apjcp.2012.13.6.2729.
Despite clinical advances in anticancer therapy, there is still a need for novel anticancer metabolites, with higher efficacy and lesser side effects. Oroxylum indicum (L.) Vent. is a small tree of the Bignoniaceae family which is well known for its food and medicinal properties. In present study, the chemopreventive properties of O. indicum hot and cold non-polar extracts (petroleum ether and chloroform) were investigated with MDA- MB-231 (cancer cells) and WRL-68 (non-tumor cells) by XTT assay. All the extracts, and particularly the petroleum ether hot extract (PHO), exhibited significantly (P<0.05) higher cytotoxicity in MDA-MB-231 when compared to WRL-68 cells. PHO was then tested for apoptosis induction in estrogen receptor (ER)-negative (MDA-MB-231) and ER-positive (MCF-7) breast cancer cells by cellular DNA fragmentation ELISA, where it proved more efficient in the MDA-MB-231 cells. Further, when PHO was tested for anti-metastatic potential in a cell migration inhibition assay, it exhibited beneficial effects. Thus non-polar extracts of O. indicum (especially PHO) can effectively target ER-negative breast cancer cells to induce apoptosis, without harming normal cells by cancer-specific cytotoxicity. Hence, it could be considered as an extract with candidate precursors to possibly harness or alleviate ER-negative breast cancer progression even in advanced stages of malignancy.
尽管抗癌治疗在临床上取得了进展，但仍需要新型抗癌代谢物，其疗效更高且副作用更小。木蝴蝶是紫葳科的一种小树，以其食用和药用特性而闻名。在本研究中，通过XTT试验，用MDA-MB-231（癌细胞）和WRL-68（非肿瘤细胞）研究了木蝴蝶热和冷非极性提取物（石油醚和氯仿）的化学预防特性。与WRL-68细胞相比，所有提取物，特别是石油醚热提取物（PHO），在MDA-MB-231中表现出显著更高的细胞毒性（P<0.05）。然后通过细胞DNA片段化ELISA检测PHO对雌激素受体（ER）阴性（MDA-MB-231）和ER阳性（MCF-7）乳腺癌细胞的凋亡诱导作用，结果表明其在MDA-MB-231细胞中更有效。此外，当在细胞迁移抑制试验中检测PHO的抗转移潜力时，它表现出有益的效果。因此，木蝴蝶的非极性提取物（尤其是PHO）可以有效地靶向ER阴性乳腺癌细胞以诱导凋亡，而不会因癌症特异性细胞毒性而损害正常细胞。因此，即使在恶性肿瘤的晚期，它也可被视为一种可能用于控制或缓解ER阴性乳腺癌进展的候选前体提取物。
