Brown O R, Boehme D, Yein F
Microbios. 1978;23(93-94):175-92.
The growth-inhibitory effect of 4.2 atm of hyperbaric oxygen for Escherichia coli was strongly influenced by available nutrients. A pattern of protection was achieved with various carbohydrate intermediates which was consistent with oxygen-induced poisoning of fructose-1,6-diphosphatase and of enzymes required in the pentose shunt and for converting galactose into glucose. Two of these sites have not been investigated further, but direct evidence was obtained that purified fructose-1,6-diphosphatase was inactivated in vitro by superoxide anion, but not by molecular oxygen at hyperbaric pressure (4.2 atm). Poisioning of fructose-1,6-diphosphatase by metabolically generated oxygen radicals, such as superoxide ion, would have deleterious effects for E. coli in media where synthesis of glucose by reverse glycolysis is required, and presumably for cells of higher organisms, including man.
4.2个大气压的高压氧对大肠杆菌的生长抑制作用受到可用营养物质的强烈影响。各种碳水化合物中间体形成了一种保护模式，这与氧诱导的果糖-1,6-二磷酸酶以及戊糖磷酸途径和将半乳糖转化为葡萄糖所需的酶中毒一致。其中两个位点尚未进一步研究，但已获得直接证据表明，纯化的果糖-1,6-二磷酸酶在体外被超氧阴离子灭活，而不是在高压（4.2个大气压）下被分子氧灭活。代谢产生的氧自由基（如超氧离子）对果糖-1,6-二磷酸酶的毒害作用，对于需要通过逆向糖酵解合成葡萄糖的培养基中的大肠杆菌，以及大概对于包括人类在内的高等生物的细胞，都会产生有害影响。
