Virology Division, SEALS Microbiology, Prince of Wales Hospital, Randwick, NSW 2031, Australia.
J Virol Methods. 2012 Dec;186(1-2):109-12. doi: 10.1016/j.jviromet.2012.08.008. Epub 2012 Aug 29.
The presence of viral respiratory infections is associated closely with exacerbations in patients with cystic fibrosis. Viral and bacterial multiplex PCRs were developed and applied to nasal swab samples from children with cystic fibrosis. This showed a large number of individuals with cystic fibrosis were infected with rhinoviruses, and more were infected with viral than bacterial pathogens. All individuals with parainfluenza 3 virus had clinical exacerbations of their cystic fibrosis, and although 3/4 of these children were co-infected with HRV. The findings do not suggest a significant association for any other virus or bacteria with exacerbation. There is clear evidence some viral infections are associated with cystic fibrosis that dual infection is more likely to produce symptoms, and mechanisms of viral-induced exacerbation should be elucidated.
病毒呼吸道感染的存在与囊性纤维化患者的恶化密切相关。我们开发并应用了病毒和细菌多重 PCR 来检测囊性纤维化儿童的鼻拭子样本。结果表明，大量囊性纤维化患者感染了鼻病毒，而且感染病毒的人数多于细菌病原体。所有感染副流感 3 型病毒的患者都出现了囊性纤维化的临床恶化，尽管其中 3/4 的患者同时感染了 HRV。这些发现并没有表明任何其他病毒或细菌与恶化有显著关联。有明确的证据表明，一些病毒感染与囊性纤维化有关，双重感染更有可能产生症状，因此应该阐明病毒引起恶化的机制。
