Department of Microbiology, Goa University, Taleigao Plateau, Goa 403 206, India.
Bioresour Technol. 2012 Nov;123:256-62. doi: 10.1016/j.biortech.2012.07.056. Epub 2012 Jul 27.
Pseudomonas nitroreducens TSB.MJ10 exhibiting growth and bioemulsifier production with 0.5% sodium benzoate as the sole carbon source was isolated from a mangrove ecosystem in the vicinity of a petroleum pump. The bioemulsifier is a lipopeptide that is stable over a pH range of 5-11 and a temperature range of 20-90°C and showed emulsifying activity in the presence of relatively high NaCl concentrations (up to 25%). The bioemulsifier formed stable emulsions with aliphatic (hexadecane, n-heptane, cyclohexane), aromatic (xylene, benzene, toluene) and petroleum (gasoline, diesel, kerosene, crude oil) compounds. It exhibited a maximum emulsification activity with weathered crude oil (97%) and was capable of transforming the rheological behavior of the pseudoplastic to a Newtonian fluid. The results reveal the potential of the bioemulsifier for use in bioremediation of hydrocarbons in marine environments and in enhanced oil recovery.
从附近石油泵站的红树林生态系统中分离出 Pseudomonas nitroreducens TSB.MJ10，它能以 0.5%的苯甲酸钠作为唯一碳源生长并产生生物乳化剂。该生物乳化剂是一种脂肽，在 pH 值为 5-11 和温度范围为 20-90°C 的条件下稳定，并在相对高的 NaCl 浓度（高达 25%）存在下表现出乳化活性。该生物乳化剂能与脂肪族（十六烷、正庚烷、环己烷）、芳香族（二甲苯、苯、甲苯）和石油（汽油、柴油、煤油、原油）化合物形成稳定的乳液。它对风化原油（97%）表现出最大的乳化活性，并且能够改变假塑性流变行为为牛顿流体。这些结果表明该生物乳化剂在海洋环境中烃类生物修复和提高采油率方面具有应用潜力。
