School of Civil Engineering, Southeast University, Nanjing, Jiangsu 210096, China; Lawrence Berkeley National Laboratory, Berkeley, CA, United States; Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720-3102, United States.
School of Civil Engineering, Southeast University, Nanjing, Jiangsu 210096, China.
Bioresour Technol. 2019 Jun;282:456-463. doi: 10.1016/j.biortech.2019.01.131. Epub 2019 Jan 29.
The aim of this work was to study the production of bioemulsifier by Rhodococcus erythropolis OSDS1, and the improvement of crude oil depletion efficiency using a consortium of petroleum hydrocarbon degraders and OSDS1. The results showed that R. erythropolis OSDS1 produced highly stable bioemulsifier under various salinity (0-35 g/L NaCl) and pH (5.0-9.0) conditions; more than 90% of the initial emulsification activity was retained after 168 h. Emulsification capacity of the bioemulsifier on different petroleum hydrocarbons was diesel > mineral oil/crude oil > gasoline. A mixed bacterial consortium combining OSDS1 and four other petroleum hydrocarbon degraders was constructed. GC-MS results revealed that the constructed consortium achieved 85.26% depletion efficiency of crude oil in 15 days, which was significantly higher than that of individual strains. During the process, alkane hydroxylase gene (alkB) was successfully amplified from the consortium, confirming presence of crude oil degrading enzymes.
本工作旨在研究红球菌 OSDS1 产生生物乳化剂的情况，并利用石油烃降解菌和 OSDS1 的混合物提高原油枯竭效率。结果表明，R. erythropolis OSDS1 在各种盐度（0-35g/L NaCl）和 pH 值（5.0-9.0）条件下均可产生高度稳定的生物乳化剂；经过 168 小时后，初始乳化活性保持在 90%以上。该生物乳化剂对不同石油烃的乳化能力为柴油＞矿物油/原油＞汽油。构建了一个结合 OSDS1 和其他四种石油烃降解菌的混合细菌共生体。GC-MS 结果表明，构建的共生体在 15 天内实现了 85.26%的原油枯竭效率，明显高于单一菌株。在此过程中，成功从共生体中扩增出烷烃羟化酶基因（alkB），证实了存在原油降解酶。
