Graduate School of Science and Technology, Kobe University, 3-11 Tsurukabuto, Nada-ku, Kobe 657-8501, Japan.
Bioorg Med Chem Lett. 2012 Oct 1;22(19):6139-43. doi: 10.1016/j.bmcl.2012.08.028. Epub 2012 Aug 15.
Novel trigonal DNA-carbohydrate conjugates were prepared and evaluated to explore efficient carbohydrate-lectin interactions. Carbohydrate-modified oligonucleotides were enzymatically prepared, then hybridized to form 3-way junction DNAs. The thermal stabilities of the junctions were assessed by UV melting analysis and formation of constructs was confirmed by gel electrophoresis. Fluorescence titration assays revealed that the trigonal DNA-carbohydrate conjugates exhibit high affinity to lectins depending on the distribution of carbohydrates presented in each arm. These results suggest that self-assembled 3-way DNA architectures could offer a useful platform for controlling the spatial distribution of carbohydrates on conjugates and achieving more efficient molecular recognition.
新型三角型 DNA-碳水化合物缀合物被制备并加以评估，以探索有效的碳水化合物-凝集素相互作用。通过酶法制备碳水化合物修饰的寡核苷酸，然后杂交形成 3 路连接 DNA。通过紫外熔融分析评估连接的热稳定性，并通过凝胶电泳确认构建体的形成。荧光滴定实验表明，根据每个臂中呈现的碳水化合物的分布，三角型 DNA-碳水化合物缀合物与凝集素有高亲和力。这些结果表明，自组装的 3 路 DNA 结构可以为控制缀合物上碳水化合物的空间分布和实现更有效的分子识别提供一个有用的平台。
