通过SELMA对靶向2G12的九甘露糖糖簇进行定向进化。
Directed evolution of 2G12-targeted nonamannose glycoclusters by SELMA.
作者信息
Temme J Sebastian, Drzyzga Michael G, MacPherson Iain S, Krauss Isaac J
机构信息
Department of Chemistry, Brandeis University, 415 South St., Waltham, MA 02454 (USA).
出版信息
Chemistry. 2013 Dec 16;19(51):17291-5. doi: 10.1002/chem.201303848. Epub 2013 Nov 13.
Abstract
Clusters of Man glycans which are recognized by broadly-neutralizing anti-HIV antibody 2G12 have potential as HIV vaccines. However, optimal recognition by 2G12 requires optimal clustering of glycans. Using our recently described SELMA technique (SELection with Modified Aptamers), we have developed Man clusters in which glycans are supported by DNA sequences selected from among 2 × 10 variants.
摘要
可被广谱中和抗HIV抗体2G12识别的人聚糖簇具有作为HIV疫苗的潜力。然而，2G12的最佳识别需要聚糖的最佳聚类。利用我们最近描述的SELMA技术（用修饰适体进行选择），我们开发了聚糖由从2×10个变体中选择的DNA序列支持的甘露糖簇。
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