Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, 107 W. Wenhua Road, Jinan, 250012, P R China.
Gynecol Oncol. 2012 Dec;127(3):595-600. doi: 10.1016/j.ygyno.2012.08.028. Epub 2012 Aug 29.
The aim of this study is to evaluate the detection of paired box gene 8 (PAX8) and p53 with immunohistochemistry in pelvic washing cell block sections.
A total of 92 cases were used in this study, which were assigned to three groups according to the cytopathology files. The first group with positive cytology including endometrial and ovarian carcinomas comprised 32 cases. The second group with suspicious cytology for endometrial or ovarian carcinomas consisted of 29 cases. The third group with negative cytology (regarded as mesothelial cells) included 31 cases. The pelvic washing cell blocks underwent immunohistochemistry to detect PAX8 and p53 expression.
Immunoreactivity for PAX8 was found in 75% (24/32) of the cases in the group with positive cytology, in 6.9% (2/29) of the cases with suspicious cytology, and in none of the 31 cases with negative cytology (sensitivity: 75%; specificity: 100%; p<0.05). p53 expression was detected in 37.5% (12/32) of the cases in the first group, in 3.4% (1/29) of the cases in the second group, and in none of the cases in the third group (sensitivity: 37.5%; specificity: 100%; p<0.05). Moreover, the combined expression of PAX8 and p53 showed the same result as the single expression of p53 in the three groups.
The detection of PAX8 and p53 is beneficial in recognizing metastatic carcinomas in pelvic washings, especially in cases with suspicious cytology, which additionally supports the Müllerian origin of these carcinomas.
本研究旨在评估配对盒基因 8（PAX8）和 p53 的免疫组织化学检测在盆腔冲洗细胞块切片中的应用。
本研究共纳入 92 例病例，根据细胞学档案分为三组。第一组为细胞学阳性的病例，包括子宫内膜癌和卵巢癌，共 32 例；第二组为可疑的子宫内膜癌或卵巢癌病例，共 29 例；第三组为细胞学阴性（视为间皮细胞）病例，共 31 例。对盆腔冲洗细胞块进行免疫组织化学检测，以检测 PAX8 和 p53 的表达情况。
在细胞学阳性的病例中，PAX8 免疫反应性在 75%（24/32）的病例中阳性，在可疑的细胞学病例中为 6.9%（2/29），在细胞学阴性的病例中均为阴性（敏感性：75%；特异性：100%；p<0.05）。p53 表达在第一组的 37.5%（12/32）的病例中阳性，在第二组的 3.4%（1/29）的病例中阳性，在第三组的病例中均为阴性（敏感性：37.5%；特异性：100%；p<0.05）。此外，PAX8 和 p53 的联合表达与 p53 的单表达在三组中的结果相同。
PAX8 和 p53 的检测有助于识别盆腔冲洗液中的转移性癌，尤其是在可疑的细胞学病例中，这进一步支持了这些癌来源于 Müllerian 系统。
