Department of Frontier Surgery, Chiba University Graduate School of Medicine, Chiba, Japan.
Int J Oncol. 2012 Nov;41(5):1653-61. doi: 10.3892/ijo.2012.1614. Epub 2012 Aug 30.
The expression of microRNA-203 (miR-203) in esophageal squamous cell carcinoma (ESCC) tissues is remarkably lower than that in non‑ESCC tissues. We investigated how miR-203 could influence the development of ESCC cells. Our analyses revealed that miR-203 inhibited the migration and invasion of ESCC cells. Genome-wide gene expression data and target site inhibition assays showed that miR-203 appears to directly regulate LIM and SH3 protein 1 (LASP1). The knockdown of LASP1 resulted in inhibition of the migration and invasion of ESCC cells. Our results suggest that miR-203 and its target LASP1, may be associated with the progression of ESCC. In clinical ESCC specimens, the expression levels of miR-203, which were lower compared to those in normal tissues, were inversely correlated with the mRNA expression levels of LASP1. Moreover, we found that there was a significant correlation between the expression levels of miR-203 and the relapse‑free survival. The identification of a cancer network regulated by miR-203 could provide new insights into the potential mechanisms of the progression of ESCC.
miR-203（微小 RNA-203）在食管鳞状细胞癌（ESCC）组织中的表达明显低于非 ESCC 组织。我们研究了 miR-203 如何影响 ESCC 细胞的发展。我们的分析表明，miR-203 抑制 ESCC 细胞的迁移和侵袭。全基因组基因表达数据和靶位点抑制试验表明，miR-203 似乎直接调节 LIM 和 SH3 蛋白 1（LASP1）。LASP1 的敲低导致 ESCC 细胞迁移和侵袭的抑制。我们的结果表明，miR-203 及其靶标 LASP1 可能与 ESCC 的进展有关。在临床 ESCC 标本中，miR-203 的表达水平低于正常组织，与 LASP1 的 mRNA 表达水平呈负相关。此外，我们发现 miR-203 的表达水平与无复发生存之间存在显著相关性。鉴定受 miR-203 调控的癌症网络可能为 ESCC 进展的潜在机制提供新的见解。
