Hsa_circ_0004370 通过 miR-1294/LASP1 通路促进食管癌进展。
Hsa_circ_0004370 promotes esophageal cancer progression through miR-1294/LASP1 pathway.
机构信息
Department of Thoracic Surgery, Fujian Medical University Union Hospital, Fuzhou City, 350001 Fujian Province, China.
Department of Thoracic Surgery, Fujian Medical University Union Hospital, Fuzhou City, 350001 Fujian Province, China
出版信息
Biosci Rep. 2019 May 14;39(5). doi: 10.1042/BSR20182377. Print 2019 May 31.
Circular RNAs (circRNAs) formed by back-splicing play multiple roles in the occurrence and development of cancer. Here, we found that hsa_circ_0004370 was up-regulated in both esophageal cancer (EC) tissues and cell lines. Loss function of hsa_circ_0004370 by si-RNA significantly suppressed proliferation and invasion and promoted apoptosis in both EC cell lines. The sponging of miR-1294 by hsa_circ_0004370 was bioinformatically predicted and subsequently verified by luciferase reporter assay and RNA immunoprecipitation assay. Further, hsa_circ_0004370 involved in the up-regulation of LASP1 by sponging miR-1294. Besides, the inhibition of the down-regulated hsa_circ_0004370 on cell malignant behaviors was rescued by miR-1294 inhibitor. Finally, this rescue effect was abrogated by suppressing the expression of LASP1. The results present here suggest that hsa_circ_0004370 functions as an oncogene on cell proliferation, apoptosis, and invasion via miR-1294/LASP1 axis.
环形 RNA（circRNAs）通过反向剪接形成，在癌症的发生和发展中发挥多种作用。在这里，我们发现 hsa_circ_0004370 在食管癌（EC）组织和细胞系中均上调。通过 si-RNA 敲低 hsa_circ_0004370 的功能显著抑制了两种 EC 细胞系的增殖和侵袭，并促进了细胞凋亡。hsa_circ_0004370 通过生物信息学预测与 miR-1294 结合，随后通过荧光素酶报告基因检测和 RNA 免疫沉淀实验得到验证。此外，hsa_circ_0004370 通过海绵吸附 miR-1294 参与 LASP1 的上调。此外，miR-1294 抑制剂可挽救下调 hsa_circ_0004370 对细胞恶性行为的抑制作用。最后，通过抑制 LASP1 的表达，消除了这种挽救作用。这些结果表明 hsa_circ_0004370 通过 miR-1294/LASP1 轴在细胞增殖、凋亡和侵袭中发挥致癌基因的作用。
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