亚急性硬化性全脑炎和多发性硬化症患者脑脊液和血清中的麻疹病毒特异性免疫球蛋白D抗体。

Measles virus-specific immunoglobulin D antibody in cerebrospinal fluid and serum from patients with subacute sclerosing panencephalitis and multiple sclerosis.

作者信息

Patrick B A, Mehta P D, Sobczyk W, Kulczycki J, Woyciechowska-Camenga J, Camenga D, Thormar H

机构信息

Department of Virology, New York State Institute for Basic Research in Developmental Disabilities, Staten Island 10314.

出版信息

J Neuroimmunol. 1990 Jan;26(1):69-74. doi: 10.1016/0165-5728(90)90121-3.

DOI:10.1016/0165-5728(90)90121-3

PMID:2294143

Abstract

Quantitation of measles-specific immunoglobulin D (IgD) antibody was carried out in cerebrospinal fluid (CSF) and serum samples from 18 patients with subacute sclerosing panencephalitis (SSPE), 12 patients with multiple sclerosis (MS) and seven normal controls with high measles antibody titers in serum, using polyclonal and monoclonal antibodies specific for human IgD and enzyme-linked immunosorbent assay. Measles-specific IgD activity was significantly higher in CSF and serum from SSPE patients compared to that found in patients with MS or normal controls. The IgD antibody to measles virus was not due to high levels of measles-specific IgG since significant measles IgD activity was found after eluting IgG from SSPE serum. The increased level of measles-specific IgD found in SSPE sera is consistent with the levels observed in patients with acute and chronic viral infections.

摘要

采用针对人IgD的多克隆和单克隆抗体以及酶联免疫吸附测定法，对18例亚急性硬化性全脑炎（SSPE）患者、12例多发性硬化症（MS）患者的脑脊液（CSF）和血清样本，以及7名血清中麻疹抗体滴度高的正常对照者的样本进行了麻疹特异性免疫球蛋白D（IgD）抗体定量检测。与MS患者或正常对照者相比，SSPE患者脑脊液和血清中的麻疹特异性IgD活性显著更高。麻疹病毒的IgD抗体并非由于麻疹特异性IgG水平过高所致，因为从SSPE血清中洗脱IgG后仍发现有显著的麻疹IgD活性。在SSPE血清中发现的麻疹特异性IgD水平升高与急性和慢性病毒感染患者中观察到的水平一致。