Conrad A J, Chiang E Y, Andeen L E, Avolio C, Walker S M, Baumhefner R W, Mirzayan R, Tourtellotte W W
Department of Neurology, UCLA School of Medicine 90024.
J Neuroimmunol. 1994 Oct;54(1-2):99-108. doi: 10.1016/0165-5728(94)90236-4.
A method for quantitating specific anti-viral antibodies in serum and cerebrospinal fluid (CSF) is established using enzyme-linked immunosorbent assay (ELISA). Quantitated antibody levels are used to determine intrathecal specific IgG synthesis rate for the particular antibody. Measles virus was used as a model for validating this quantitative technique: a mutated form of measles virus is a cause of subacute sclerosing panencephalitis (SSPE) and there is a possibility that measles virus is related to the cause of multiple sclerosis (MS). Matched serum and CSF samples were assayed. Concentration of anti-measles IgG was determined and intrathecal measles-specific IgG synthesis rate was calculated. For the SSPE samples, measles-specific IgG synthesis rate was elevated and comprised > 20% of the total intrathecal IgG synthesis rate; these results are consistent with the literature. The ELISA method can be performed routinely, providing a quick, simple, reproducible means of quantitating specific antibody concentrations, with sensitivity greater than 1 nanogram per milliliter. With this method, quantitation of IgG antibodies to any other viral antigen can be reliably and precisely determined.
采用酶联免疫吸附测定法（ELISA）建立了一种定量血清和脑脊液（CSF）中特异性抗病毒抗体的方法。定量的抗体水平用于确定特定抗体的鞘内特异性IgG合成率。麻疹病毒被用作验证该定量技术的模型：麻疹病毒的一种突变形式是亚急性硬化性全脑炎（SSPE）的病因，并且麻疹病毒有可能与多发性硬化症（MS）的病因有关。对匹配的血清和脑脊液样本进行了检测。测定了抗麻疹IgG的浓度，并计算了鞘内麻疹特异性IgG合成率。对于SSPE样本，麻疹特异性IgG合成率升高，占鞘内总IgG合成率的>20%；这些结果与文献一致。ELISA方法可常规进行，提供了一种快速、简单、可重复的定量特异性抗体浓度的方法，灵敏度大于每毫升1纳克。使用该方法，可以可靠且精确地测定针对任何其他病毒抗原的IgG抗体的定量。
