Iwaki T, Kume-Iwaki A, Goldman J E
Department of Pathology, Columbia University College of Physicians and Surgeons, New York, NY 10032.
J Histochem Cytochem. 1990 Jan;38(1):31-9. doi: 10.1177/38.1.2294148.
alpha B-Crystallin is a subunit of alpha-crystallin, a major protein component of the vertebrate lens. Recently, its expression in various extra-lenticular tissues has been demonstrated by both Western and Northern blotting. In this study, the cellular distribution of alpha B-crystallin in rat organs was examined in detail using immunohistochemistry. Positive reactions were observed in lens, iris, heart, skeletal muscle (type 1 and type 2A fibers), striated muscle in skin and esophagus, Henle's loop and medullary collecting duct of the kidney, Schwann cells of peripheral nerves, glia of the central nervous system, and decidual cells of the placenta. A close correlation with markers of oxidative activity suggests that alpha B-crystallin is expressed in cells that have high levels of oxidative function.
αB-晶状体蛋白是α-晶状体蛋白的一个亚基，α-晶状体蛋白是脊椎动物晶状体的主要蛋白质成分。最近，通过蛋白质免疫印迹法和Northern印迹法均已证明其在各种晶状体外组织中的表达。在本研究中，使用免疫组织化学详细检查了大鼠器官中αB-晶状体蛋白的细胞分布。在晶状体、虹膜、心脏、骨骼肌（1型和2A型纤维）、皮肤和食管的横纹肌、肾脏的髓袢和髓质集合管、周围神经的施万细胞、中枢神经系统的神经胶质细胞以及胎盘的蜕膜细胞中观察到阳性反应。与氧化活性标志物的密切相关性表明αB-晶状体蛋白在具有高水平氧化功能的细胞中表达。
