Iwaki T, Iwaki A, Liem R K, Goldman J E
Department of Pathology, Columbia University College of Physicians and Surgeons, New York, New York.
Kidney Int. 1991 Jul;40(1):52-6. doi: 10.1038/ki.1991.178.
The expression and cellular localization of alpha B-crystallin during rat renal development was studied by Northern blot analysis and by immunocytochemistry. Northern blotting of total RNA extracted from whole kidneys revealed that the messenger RNA for alpha B-crystallin rapidly increased after birth to reach adult levels by 20 days. At the same time, immunohistochemistry for alpha B-crystallin demonstrated that the prominent elongation of Henle's loop during the first 10 days of life was accompanied by increased alpha B-crystallin expression. Thus, the development of alpha B-crystallin expression is correlated temporally with the acquisition of tubule function in early post-natal life.
通过Northern印迹分析和免疫细胞化学研究了αB-晶状体蛋白在大鼠肾脏发育过程中的表达及细胞定位。对从全肾提取的总RNA进行Northern印迹分析显示，αB-晶状体蛋白的信使RNA在出生后迅速增加，到20天时达到成年水平。同时，αB-晶状体蛋白的免疫组织化学表明，出生后前10天亨利氏袢的显著延长伴随着αB-晶状体蛋白表达的增加。因此，αB-晶状体蛋白表达的发育在时间上与出生后早期肾小管功能的获得相关。
