Department of Chemistry, College of Science, Sultan Qaboos University, Box 36, Al-Khod, 123, Oman.
Luminescence. 2013 Sep-Oct;28(5):618-27. doi: 10.1002/bio.2418. Epub 2012 Sep 3.
This review will discuss various approaches and techniques in which analysis using microfluidics-chemiluminescence systems (MF-CL) has been reported. A variety of applications is examined, including environmental, pharmaceutical, biological, food and herbal analysis. Reported uses of CL reagents, sample introduction techniques, sample pretreatment methods, CL signal enhancement and detection systems are discussed. A hydrodynamic pumping system is predominately used for these applications. However, several reports are available in which electro-osmotic (EO) pumping has been implemented. Various sample pretreatment methods have been used, including liquid-liquid extraction, solid-phase extraction and molecularly imprinted polymers. A wide range of innovative techniques has been reported for CL signal enhancement. Most of these techniques are based on enhancement of the mixing process in the microfluidics channels, which leads to enhancement of the CL signal. However, other techniques are also reported, such as mirror reaction, liquid core waveguide, on-line pre-derivatization and the use of an opaque white chip with a thin transparent seal. Photodetectors are the most commonly used detectors; however, other detection systems have also been used, including integrated electrochemiluminescence (ECL) and organic photodiodes (OPDs).
本文综述了基于微流控-化学发光（MF-CL）系统的各种分析方法和技术。考察了包括环境、制药、生物、食品和草药分析在内的多种应用。讨论了报道的 CL 试剂、样品引入技术、样品预处理方法、CL 信号增强和检测系统的用途。这些应用主要采用流体动力泵系统，但也有一些电渗（EO）泵的应用报道。已经使用了各种样品预处理方法，包括液-液萃取、固相萃取和分子印迹聚合物。还报道了各种用于 CL 信号增强的创新技术。这些技术大多基于增强微流控通道中的混合过程，从而增强 CL 信号。然而，也有其他技术的报道，例如镜反应、液芯波导、在线预衍生化以及使用具有薄透明密封的不透明白色芯片。光电探测器是最常用的检测器，但也使用了其他检测系统，包括集成电化学发光（ECL）和有机光电二极管（OPDs）。
