Research Center of Biosensors and Medical Instruments, Shenzhen Institutes of Advanced Technology, CAS, Shenzhen, China.
Appl Biochem Biotechnol. 2012 Feb;166(3):786-95. doi: 10.1007/s12010-011-9467-9. Epub 2011 Dec 3.
We have demonstrated an integrated platform for microfluidics and chemiluminescence (CL) detection that is capable of parallel cell culture, convenient liquid manipulation, and sensitive chemiluminescent detection. Luminol-dependent CL responses induced by three different stimuli, phytohemagglutinin (PHA), concanavalin A (ConA), and lipopolysaccharides (LPS), which can evoke a CL response in macrophages, were evaluated on this microfluidic chip. We studied the dose-dependent effect of these three stimuli on CL response in murine macrophages. PHA produced the highest CL response compared to LPS and ConA. The CL intensity produced by PHA was 6.85 and four times higher than that by LPS and ConA, respectively, at the low concentration of 100 μg/ml. We have found microfluidic based CL to be a very useful screening tool, which is less laborious and more sensitive. This microfluidic system is disposable and capable of rapid device prototyping; it may prove to be very useful in clinical and pharmaceutical applications.
我们展示了一种集成的微流控和化学发光（CL）检测平台，该平台能够进行平行细胞培养、方便的液体操作和灵敏的化学发光检测。在这个微流控芯片上评估了三种不同刺激物（植物血球凝集素（PHA）、刀豆球蛋白 A（ConA）和脂多糖（LPS））诱导的依赖鲁米诺的 CL 反应，这些刺激物可以在巨噬细胞中引发 CL 反应。我们研究了这三种刺激物对细胞 CL 反应的剂量依赖性影响。与 LPS 和 ConA 相比，PHA 产生的 CL 反应最高。在低浓度 100μg/ml 时，PHA 产生的 CL 强度分别比 LPS 和 ConA 高 6.85 倍和 4 倍。我们发现基于微流控的 CL 是一种非常有用的筛选工具，它不那么费力，而且更灵敏。这种微流控系统是一次性的，能够快速进行设备原型制作；它在临床和制药应用中可能非常有用。
