Department of Phytopharmacy and Phytomedicine, (TIFAC CORE HD), JSS Medical College, Mysore, India.
J Pharm Pharmacol. 2012 Oct;64(10):1501-9. doi: 10.1111/j.2042-7158.2012.01528.x. Epub 2012 May 14.
This study involves the evaluation of Memecylon malabaricum Cogn. (Melastomataceae) leaves for antipsoriatic activity.
Aqueous extract, hydroalcoholic extract and their fractions of M. malabaricum leaves were evaluated for in-vivo antipsoriatic activity by mouse tail test and for in-vitro antipsoriatic activity using HaCaT cells, lipoxygenase inhibition and thymidine phosphorylase inhibition assays. Extracts and fractions were evaluated for total phenol and flavonoid contents. HPTLC was used for screening and fingerprint analysis of the extracts and active fraction.
M. malabaricum hydroalcoholic extract (MMHA) and water fraction of MMHA (MMHAW) produced significant (P<0.05) percent orthokeratosis in the mouse tail test. All samples except MMHA showed a significant (P < 0.05) reduction in epidermal thickness in the mouse tail test when compared with control. Maximum activity against HaCaT cells was shown by chloroform fraction of MMHA (MMHAC). The M. malabaricum decoction (MMD) and water fraction of MMD (MMDW) showed equally good inhibition of lipoxygenase. In thymidine phosphorylase inhibition assay only MMD showed activity.
The findings of this investigation reveal that the leaves of M. malabaricum have good antipsoriatic potential, which provides scope for further detailed research in to this plant for psoriasis.
本研究评估了长隔木（野牡丹科）叶的抗银屑病活性。
通过小鼠尾试验评估长隔木叶的水提物、水醇提物及其馏分的体内抗银屑病活性，通过 HaCaT 细胞、脂氧合酶抑制和胸苷磷酸化酶抑制试验评估体外抗银屑病活性。提取物和馏分的总酚和类黄酮含量进行评估。HPTLC 用于提取物和活性馏分的筛选和指纹分析。
长隔木水醇提物（MMHA）和 MMHA 的水馏分（MMHAW）在小鼠尾试验中产生了显著的（P<0.05）正角化百分率。与对照组相比，所有样本除 MMHA 外，在小鼠尾试验中均显示出表皮厚度的显著（P<0.05）减少。MMHA 的氯仿馏分（MMHAC）对 HaCaT 细胞表现出最大的活性。MMD 和 MMD 的水馏分（MMDW）对脂氧合酶显示出同样良好的抑制作用。在胸苷磷酸化酶抑制试验中，只有 MMD 显示出活性。
本研究结果表明，长隔木的叶子具有良好的抗银屑病潜力，为进一步深入研究该植物治疗银屑病提供了依据。
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