驱虫斑鸠菊果脂肪酸富集部位的抗银屑病作用。
Antipsoriatic effect of fatty acid enriched fraction of Vernonia anthelmintica Willd. fruits.
机构信息
Natural Product Chemistry and Process Development Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, Himachal Pradesh, India; Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147002, Punjab, India.
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147002, Punjab, India.
出版信息
J Ethnopharmacol. 2018 Oct 5;224:85-90. doi: 10.1016/j.jep.2018.05.038. Epub 2018 May 26.
ETHNOPHARMACOLOGICAL RELEVANCE
Vernonia anthelmintica has been utilized conventionally as an ingredient in Ayurveda and traditional Uighur medicine for management of various skin ailments, and scientific data's have substantiated its use in treating vitiligo, dermatosis and leucoderma. The present investigation was focused to evaluate the antipsoriatic activity of V. anthelmintica fruit extracts and fractions.
MATERIALS AND METHODS
Ointment containing dichloromethane (DCM) and methanol (MeOH) extracts at topical dose of 2.5% and 5% (w/w) was evaluated using mouse tail model of psoriasis. Bioactivity-guided fractionation (F-F) of most active extract was carried out and fractions were again subjected to mouse tail model. Further the activity of bioactive fraction was confirmed in HaCaT (human keratinocyte) cell line using MTT (3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide) assay and its chemical characterization was done via gas chromatography mass spectrometry (GC-MS).
RESULTS
The dichloromethane extract (5%, w/w) showed statistically significant (* p < 0.05) antipsoriatic activity (66.97 ± 2.68%) with respect to control (25.45 ± 1.80%) and equivalent to that of the standard drug, retino-A 0.05%, (72.47 ± 2.14%) in terms of degree of orthokeratosis, whereas methanol extract (5%, w/w) showed significant (* p < 0.05) differentiation (45.86 ± 2.02%) in comparison to the control group. Out of all fractions, F showed statistically significant (* p < 0.05) antipsoriatic activity (69.27 ± 2.76%) with respect to control and equivalent to that of the standard. F (15.6-1000 µg/ml) showed dose-dependent inhibition of HaCaT cell lines proliferation which suggests keratinocyte modulating activity of V. anthelmintica. Chemical characterization of F revealed that essential fatty acids (i.e., linoleic acid, palmitic acid, oleic acid and stearic acid) formed the bulk of bioactive fraction.
CONCLUSION
Ameliorative effect of V. anthelmintica in psoriasis might be attributed to the presence of essential fatty acids and thus corroborates its traditional use in the treatment of skin ailments.
民族药理学相关性
在印度阿育吠陀和传统维吾尔医学中， Vernonia anthelmintica 被传统地用作治疗各种皮肤疾病的成分，科学数据证实其在治疗白癜风、皮肤病和白斑病方面的用途。本研究旨在评估 Vernonia anthelmintica 果实提取物和馏分的抗银屑病活性。
材料和方法
使用 2.5%和 5%（w/w）的局部剂量的二氯甲烷（DCM）和甲醇（MeOH）提取物的软膏，在银屑病小鼠尾巴模型中进行评估。对最活跃的提取物进行基于生物活性的分级（F-F），并再次将馏分用于小鼠尾巴模型。进一步在 HaCaT（人角质形成细胞）细胞系中使用 MTT（3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物）测定法证实生物活性馏分的活性，并通过气相色谱质谱法（GC-MS）进行其化学表征。
结果
二氯甲烷提取物（5%，w/w）在角质形成过度方面显示出统计学上显著的（*p<0.05）抗银屑病活性（66.97±2.68%），与对照（25.45±1.80%）相当，与标准药物视黄醇-A 0.05%（72.47±2.14%）相当，而甲醇提取物（5%，w/w）与对照组相比，显示出显著的（*p<0.05）分化（45.86±2.02%）。在所有馏分中，F 显示出统计学上显著的（*p<0.05）抗银屑病活性（69.27±2.76%），与对照相当，与标准相当。F（15.6-1000μg/ml）显示出剂量依赖性的 HaCaT 细胞系增殖抑制作用，这表明 Vernonia anthelmintica 具有角质形成细胞调节活性。F 的化学表征表明，必需脂肪酸（即亚油酸、棕榈酸、油酸和硬脂酸）构成了生物活性馏分的主要成分。
结论
Vernonia anthelmintica 在银屑病中的改善作用可能归因于必需脂肪酸的存在，因此证实了其在治疗皮肤疾病方面的传统用途。
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