a Facultad de Ciencias Médicas , Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP) , CONICET-UNLP, CCT-La Plata , La Plata , Argentina.
Nat Prod Res. 2013;27(16):1475-8. doi: 10.1080/14786419.2012.718775. Epub 2012 Sep 4.
The effects of the essential oil of mandarin peel (Corrientes, Argentina) and limonene (its major component) were studied on two human tumour cell lines growth (lung adenocarcinoma A549 and hepatocarcinoma HepG2). The essential oil was obtained by cold press and its composition was investigated by gas chromatography (GC) and GC/mass spectrometry (MS) analysis. The antiproliferative effect was studied using an MTT assay. Both mandarin essential oil and limonene tested showed a strong dose-dependent effect on the growth inhibition of these cell lines. The essential oil was more effective in A549 than in HepG2 cells and more effective than limonene in both the cases. It is likely that minor components and limonene of the oil could exert additive or synergistic effects. Hence, mandarin essential oil could lead to the development of anti-tumour agent or complementary and alternative medicines for the treatment of diverse cancers.
研究了桔皮精油（阿根廷科连特斯）和柠檬烯（其主要成分）对两种人肿瘤细胞系生长（肺腺癌细胞 A549 和肝癌细胞 HepG2）的影响。通过冷榨获得精油，并通过气相色谱（GC）和 GC/质谱（MS）分析研究其组成。用 MTT 法研究了抗增殖作用。桔皮精油和柠檬烯都表现出对这些细胞系生长抑制的强烈剂量依赖性效应。在 A549 细胞中，精油比 HepG2 细胞更有效，在两种情况下都比柠檬烯更有效。油中的少量成分和柠檬烯可能具有相加或协同作用。因此，桔皮精油可能会导致抗肿瘤药物或用于治疗多种癌症的补充和替代药物的开发。
