Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine Inada-machi, Obihiro, Hokkaido, Japan.
Anim Sci J. 2012 Sep;83(9):663-7. doi: 10.1111/j.1740-0929.2012.01032.x. Epub 2012 Jun 27.
Bone morphogenetic protein-4 (BMP-4) and BMP-7, theca cell-derived growth factors, directly affect the granulosa cell function. The aim of this study was to examine the involvement of BMP-4 or BMP-7 in vascular endothelial growth factor (VEGF) expression in bovine granulosa cells. Granulosa cells were collected from small follicles (4-6 mm) and seeded at a density of 2-5 × 10(5) cells per well in Dulbecco's modified Eagle's medium (DMEM)/F12 medium with BMP-4 or BMP-7. The expression of VEGF messenger RNA and protein was the maximum when 1.0 ng/mL of BMP-4 was added to the culture medium. On the other hand, 10 ng/mL of BMP-7 significantly increased the expression of the VEGF gene and protein. In addition, BMP-4 stimulated the expression of Smad1 and Smad5 genes in granulosa cells, whereas BMP-7 stimulated the expression of Smad5 gene. These results suggested that BMP-4 and BMP-7 may be associated with VEGF expression via several specific Smads in bovine granulosa cells: BMP-4 via Smad1/Smad5 and BMP-7 via Smad5. In conclusion, theca cell-derived BMP-4 and BMP-7 might contribute to follicular vasculature and development by inducing VEGF expression in granulosa cells.
骨形态发生蛋白 4（BMP-4）和 BMP-7 是颗粒细胞来源的生长因子，可直接影响颗粒细胞的功能。本研究旨在探讨 BMP-4 或 BMP-7 是否参与牛颗粒细胞血管内皮生长因子（VEGF）的表达。从小卵泡（4-6mm）中收集颗粒细胞，并以每孔 2-5×10(5)个细胞的密度接种于含 BMP-4 或 BMP-7 的 Dulbecco 改良 Eagle 培养基（DMEM/F12）中。当向培养基中添加 1.0ng/mL 的 BMP-4 时，VEGF 信使 RNA 和蛋白的表达达到最大值。另一方面，10ng/mL 的 BMP-7 显著增加了 VEGF 基因和蛋白的表达。此外，BMP-4 刺激颗粒细胞中 Smad1 和 Smad5 基因的表达，而 BMP-7 刺激 Smad5 基因的表达。这些结果表明，BMP-4 和 BMP-7 可能通过牛颗粒细胞中的几种特定 Smads 与 VEGF 的表达有关：BMP-4 通过 Smad1/Smad5，BMP-7 通过 Smad5。总之，颗粒细胞衍生的 BMP-4 和 BMP-7 可能通过诱导颗粒细胞中 VEGF 的表达，促进卵泡血管生成和发育。
