Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China.
Genes (Basel). 2021 Sep 28;12(10):1536. doi: 10.3390/genes12101536.
Smads are involved in a variety of biological activities by mediating bone morphogenetic protein (BMP) signals. The full-length coding sequences (CDSs) of buffalo , , and were isolated and identified through RT-PCR in this study. Their lengths are 1398 bp, 1662 bp, and 1398 bp, respectively. In silico analysis showed that their transcriptional region structures, as well as their amino acid sequences, physicochemical characteristics, motifs, conserved domains, and three-dimensional structures of their encoded proteins are highly consistent with their counterparts in the species of Bovidae. The three Smad proteins are all hydrophilic without the signal peptides and transmembrane regions. Each of them has an MH1 domain and an MH2 domain. A nuclear localization sequence was found in the MH1 domain of buffalo and . Prediction showed that the function of the three Smads is mainly protein binding, and they can interact with BMPs and their receptors. The three genes were expressed in all 10 buffalo tissues assayed, and their expression in the mammary gland, gonad, and spleen was relatively high. The results here indicate that the three buffalo Smads may be involved in the transcriptional regulation of genes in a variety of tissues.
Smads 通过介导骨形态发生蛋白（BMP）信号参与多种生物学活动。本研究通过 RT-PCR 从水牛中分离并鉴定了全长编码序列（CDS）。它们的长度分别为 1398bp、1662bp 和 1398bp。计算机分析表明，它们的转录区结构以及它们的氨基酸序列、理化特性、基序、保守域和编码蛋白的三维结构与牛科动物中的对应物高度一致。这三种 Smad 蛋白都是亲水的，没有信号肽和跨膜区。它们都有一个 MH1 结构域和一个 MH2 结构域。在水牛 Smad 和 Smad 的 MH1 结构域中发现了一个核定位序列。预测表明，这三种 Smads 的功能主要是蛋白结合，它们可以与 BMP 及其受体相互作用。这三个基因在检测的 10 个水牛组织中均有表达，在乳腺、性腺和脾脏中的表达水平相对较高。这些结果表明，这三种水牛 Smad 可能参与了多种组织中基因的转录调控。
