Fyfe G K, Panicker S, Jones R L, Wareing M
Maternal and Fetal Health Research Centre, The University of Manchester, Manchester Academic Health Science Centre, Manchester, UK.
J Obstet Gynaecol. 2012 Oct;32(7):624-9. doi: 10.3109/01443615.2012.709288.
Human placental expression of K(V)9.3, a voltage-gated K channel linked to tissue oxygenation responses, has been suggested at the messenger RNA level but tissue localisation has not been described. We aimed to: (1) produce an antibody to human K(V)9.3 and (2) assess channel expression and distribution in human placental tissue. We determined human placental protein expression and localisation using an antibody to K(V)9.3. Antibody specificity was confirmed by Western blotting. Staining was observed in syncytiotrophoblast microvillous membrane, endothelial cells (in intermediate, stem villi and chorionic plate blood vessels) and vascular smooth muscle cells (large diameter vessels only) by immunohistochemistry. Expression was unchanged in tissue from women with small-for-gestational age babies. It was concluded that K(V)9.3 is localised to human placental vascular tissues and syncytiotrophoblast.
有研究表明，在信使核糖核酸水平上，与组织氧合反应相关的电压门控钾通道K(V)9.3在人胎盘中有表达，但尚未描述其组织定位。我们的目的是：(1)制备抗人K(V)9.3抗体；(2)评估该通道在人胎盘组织中的表达和分布。我们使用抗K(V)9.3抗体确定了人胎盘蛋白的表达和定位。通过蛋白质印迹法确认了抗体的特异性。通过免疫组织化学观察到，在合体滋养层微绒毛膜、内皮细胞（在中间、干绒毛和绒毛膜板血管中）和血管平滑肌细胞（仅在大直径血管中）有染色。来自小于胎龄儿母亲的组织中的表达没有变化。得出的结论是，K(V)9.3定位于人胎盘血管组织和合体滋养层。
