College of Veterinary Medicine, Biosafety Research Institute, Chonbuk National University, 664-14, 1GA Deokjin-Dong, Deokjin-Gu, Jeonju City, Jeollabuk-Do 561-756, Republic of Korea.
Food Chem Toxicol. 2012 Nov;50(11):3941-8. doi: 10.1016/j.fct.2012.08.036. Epub 2012 Aug 24.
Most of the available drugs for the treatment of diabetes mellitus (DM) produce detrimental side effects, which has prompted an ongoing search for plant with the antidiabetic potential. The present study investigated the effect of soybean extracts fermented with Bacillus subtilis MORI, fermented soybean extracts (BTD-1) was investigated in streptozotocin (STZ)-induced diabetic rats. The possible effects of BTD-1 against hyperglycemia and free radical-mediated oxidative stress was investigated by assaying the plasma glucose level and the activity of enzymatic antioxidants, such as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and malondialdehyde (MDA). A significant increase in the levels of both plasma glucose and reactive oxygen species (ROS) was observed in the diabetic rats when compared to normal control group. After administration of BTD-1 (500 and 1000 mg/kg/day), the elevated plasma glucose level was significantly reduced while the plasma insulin level and the activities of SOD, GSH-Px, CAT and MDA were significantly increased. The results suggest that administration of BTD-1 can inhibit hyperglycemia and free radical-mediated oxidative stress. The administration of BTD-1 also inhibited the contractile response by norepinephrine (10(-10)-10(-5) M) in the presence of endothelium, and caused significant relaxation by carbachol (10(-8)-10(-5) M) in rat aorta. These findings indicate that BTD-1 improves vascular functions on STZ-induced diabetic rats. Therefore, subchronic administration of BTD-1 could prevent the functional changes in vascular reactivity in STZ-induced diabetic rats. The collective findings support that administration of BTD-1 may prevent some diabetes-related changes in vascular reactivity directly and/or indirectly due to its hypoglycaemic effect and inhibition of production of ROS.
大多数用于治疗糖尿病（DM）的药物都会产生有害的副作用，这促使人们一直在寻找具有抗糖尿病潜力的植物。本研究调查了经枯草芽孢杆菌 MORI 发酵的大豆提取物（BTD-1）对链脲佐菌素（STZ）诱导的糖尿病大鼠的影响。通过测定血浆葡萄糖水平和超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）和丙二醛（MDA）等酶抗氧化剂的活性，研究了 BTD-1 对高血糖和自由基介导的氧化应激的可能影响。与正常对照组相比，糖尿病大鼠的血浆葡萄糖水平和活性氧（ROS）水平均显著升高。给予 BTD-1（500 和 1000mg/kg/天）后，升高的血浆葡萄糖水平显著降低，而血浆胰岛素水平和 SOD、GSH-Px、CAT 和 MDA 的活性显著增加。结果表明，给予 BTD-1 可抑制高血糖和自由基介导的氧化应激。BTD-1 的给药还抑制了去甲肾上腺素（10（-10）-10（-5）M）在存在内皮的情况下引起的收缩反应，并通过卡巴胆碱（10（-8）-10（-5）M）引起大鼠主动脉的显著松弛。这些发现表明 BTD-1 改善了 STZ 诱导的糖尿病大鼠的血管功能。因此，BTD-1 的亚慢性给药可防止 STZ 诱导的糖尿病大鼠血管反应性的功能变化。综合研究结果支持，由于其降血糖作用和抑制 ROS 产生，BTD-1 的给药可能直接和/或间接预防一些与糖尿病相关的血管反应性变化。
