Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Cracow, Poland.
Spectrochim Acta A Mol Biomol Spectrosc. 2012 Oct;96:940-5. doi: 10.1016/j.saa.2012.07.103. Epub 2012 Aug 8.
The structure of proteins in a tissue can undergo changes on account of disease state such as diabetes or atherosclerosis. In this work the protein profile in atherosclerotic tissue is monitored by FT-IR imaging coupled with Hierarchical Cluster Analysis (HCA). Additionally, a model for prediction of secondary structure of proteins content based on amide I and II range is used to show the distribution of analyzed proteins. A new protein class emerged in atherosclerotic tissue in the region of the plaque and additionally the plaque was found to be strongly mixed with smooth muscle cell. The calculated secondary structure contents of proteins in atherosclerotic tissue in comparison to healthy tissue showed an increase of structures related to beta-sheet (E and T) and a decrease of helical (H) and unassigned arrangements.
组织中的蛋白质结构可能会因疾病状态（如糖尿病或动脉粥样硬化）而发生变化。在这项工作中，通过傅里叶变换红外成像结合层次聚类分析（HCA）来监测动脉粥样硬化组织中的蛋白质谱。此外，还使用基于酰胺 I 和 II 范围的预测蛋白质二级结构含量的模型来显示分析蛋白质的分布。在斑块区域出现了一种新的蛋白质类，此外还发现斑块与平滑肌细胞强烈混合。与健康组织相比，动脉粥样硬化组织中蛋白质的计算二级结构含量显示与β-折叠（E 和 T）相关的结构增加，而螺旋（H）和未分配结构减少。
